DECODING OF ARRAY SENSORS WITH MICROSPHERES
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Accused Products
Abstract
The invention relates to compositions and methods for decoding microsphere array sensors.
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Citations
63 Claims
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1-39. -39. (canceled)
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40. A method of decoding the position of bioactive agents attached to microspheres on an array substrate comprising:
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a) providing an array substrate comprising a population of microspheres comprising at least a first and a second subpopulation, wherein the microspheres of each subpopulation comprise;
i) a bioactive agent;
ii) at least a first and a second identifier binding ligand (IBL), wherein said first and second IBLs are different from said bioactive agent and wherein said IBLs are attached to said microsphere; and
b) detecting both the first and second IBLs to decode the position of each of said bioactive agents on said substrate. - View Dependent Claims (41, 43, 45)
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42. (canceled)
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44. (canceled)
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46-54. -54. (canceled)
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55. A method of decoding the position of a bioactive agent on an array substrate comprising:
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a) providing an array substrate comprising randomly distributed microspheres, wherein each microsphere comprises;
i) a bioactive agent; and
ii) a combination of different identifier binding ligands (IBLs), wherein said combination uniquely identifies said bioactive agent;
b) adding a first decoder binding ligand (DBL) to the array substrate, wherein the first DBL binds to an IBL in said combination;
c) identifying the position of the IBL that binds to the first DBL;
d) adding a second DBL to the array, wherein the second DBL binds to another IBL in said combination;
e) identifying the position of the IBL that binds to the second DBL; and
f) decoding the position of said bioactive agent on said array substrate based on the position on the array substrate of the IBLs that bind the first and the second DBLs. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63)
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Specification