Methods and reagents for surface functionalization
First Claim
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1. A method for functionalizing a substrate, comprising:
- providing a substrate;
contacting the substrate with a first reagent having at least first and second reactive functional groups such that the first reagent is operationally associated with the substrate by the first functional group thereby forming a monolayer;
associating a second reagent with the monolayer, the second reagent comprising a first functional group for association with the monolayer and at least a second functional group; and
associating a protein resistant reagent with the monolayer.
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Abstract
Reagents and a method for making arrays of affinity agents are disclosed. Methods of using the arrays of affinity agents also are disclosed. The arrays are particularly useful for high throughput drug screening and clinical diagnostics applications.
136 Citations
37 Claims
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1. A method for functionalizing a substrate, comprising:
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providing a substrate;
contacting the substrate with a first reagent having at least first and second reactive functional groups such that the first reagent is operationally associated with the substrate by the first functional group thereby forming a monolayer;
associating a second reagent with the monolayer, the second reagent comprising a first functional group for association with the monolayer and at least a second functional group; and
associating a protein resistant reagent with the monolayer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method for forming a monolayer, comprising:
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providing a substrate;
contacting the substrate with an asymmetric disulfide, having a reactive functional group and a protein resistant functional group, thereby forming a monolayer on the substrate. - View Dependent Claims (25, 26, 27)
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28. An organic thinfilm array, comprising:
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a substrate;
plural first reagents having first and second ends, the first end of the first reagents being operatively associated with the substrate;
plural second reagents, each second reagent having first and second ends and the first ends of the second reagent being operatively associated with the second end of the first reagent; and
a third reagent having a first end operatively associated with the second end of the first reagent and a second end, the third reagent comprising a protein resistant functional group. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36)
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37. An array device comprising:
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a substrate having an array of discrete array regions formed on the surface;
plural first reagents having first and second ends, the first end of the first reagents being operatively associated with the substrate;
plural second reagents, each second reagent having first and second ends and the first ends of the second reagent being operatively associated with the second end of the first reagent.
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Specification