Compositions and methods for treatment of microbial infections
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Abstract
The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.
12 Citations
40 Claims
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1-20. -20. (canceled)
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21. A method for vaccination, comprising, administering to a human or animal a composition comprising an effective amount of a bacterial lysate to induce an immune response, wherein the bacterial lysate results from lysis of at least one bacterial strain by infection with at least one bacteriophage, wherein,
a) the at least one bacterial strain is a Staphylococcus aureus (S. aureus) strain deposited with the Czech Collection of Microorganisms (CCM) having accession numbers CCM 4992, CCM 4993, CCM 4994, CCM 4995, CCM 4996, CCM 4997, or CCM 4998; - and,
b) the at least one bacteriophage is a bacteriophage deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmBH (DSM) having accession number DSM 14614, DSM 14615, or DSM 14616. - View Dependent Claims (22, 23, 24, 25, 26, 27)
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28. A method of preparing a biologically active fraction from a bacterial lysate, comprising,
a) forming a bacterial lysate, wherein the bacterial lysate results from lysis of at least one bacterial strain by infection with at least one bacteriophage, wherein i) the least one bacterial strain is a Staphylococcus aureus (S. aureus) strain deposited with the Czech Collection of Microorganisms (CCM) having accession numbers CCM 4992, CCM 4993, CCM 4994, CCM 4995, CCM 4996, CCM 4997, or CCM 4998; - and,
ii) the least one bacteriophage is a bacteriophage deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GMBH (DSM) having accession number DSM 14614, DSM 14615, or DSM 14616;
b) adsorbing at least a portion of the bacterial lysate to a chromatographic matrix;
c) eluting one or more fractions from the chromatographic matrix; and
,d) determining the biological activity of one or more fractions in an biological assay. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35)
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36. A method of preparing a bacterial lysate, wherein the bacterial lysate comprises,
a) growing at least one bacterial strain in culture to a concentration that is at least 1× - 106 cells per milliliter, wherein the least one bacterial strain is a Staphylococcus aureus (S. aureus) strain deposited with the Czech Collection of Microorganisms (CCM) having accession numbers CCM 4992, CCM 4993, CCM 4994, CCM 4995, CCM 4996, CCM 4997, or CCM 4998;
b) adding to the bacterial strain in culture sufficient amounts of at least one bacteriophage to yield a concentration of at least 1×
108 phage particles per milliliter, wherein the at least one bacteriophage is a bacteriophage deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmBH (DSM) having accession number DSM 14614, DSM 14615, or DSM 14616;
c) allowing infection and lysis to proceed for at least 3 hours; and
,d) sterilizing the lysate. - View Dependent Claims (37, 38, 39, 40)
- 106 cells per milliliter, wherein the least one bacterial strain is a Staphylococcus aureus (S. aureus) strain deposited with the Czech Collection of Microorganisms (CCM) having accession numbers CCM 4992, CCM 4993, CCM 4994, CCM 4995, CCM 4996, CCM 4997, or CCM 4998;
Specification