Methods and compositions of bioartificial kidney suitable for use in vivo or ex vivo

• US 20050238687A1
• Filed: 06/10/2005
• Published: 10/27/2005
• Est. Priority Date: 09/30/1996
• Status: Abandoned Application

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Humes, H. David
• Application Number:
  US11/149,266
• Publication Number:
  US 20050238687A1
• Time in Patent Office: Days
• US Class Current: 424/423
• CPC Class Codes: A61K 35/14 Blood; Artificial blood perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42
• CPC Class Codes: A61M 1/16 with membranes
• CPC Class Codes: A61M 1/1678 intracorporal peritoneal dialysis A61M1/28
• CPC Class Codes: A61M 1/28 Peritoneal dialysis ; Other peritoneal treatment, e.g. oxygenation
• CPC Class Codes: A61M 1/3489 by biological cells, e.g. bioreactor
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 1/3689 by biological cells
• CPC Class Codes: B01D 67/0088 Physical treatment with compounds, e.g. swelling, coating or impregnation
• CPC Class Codes: B01D 69/00 Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
• CPC Class Codes: B01D 69/141 Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00
• CPC Class Codes: C12M 25/10 Hollow fibers or tubes hollow fiber modules in general B01D63/02
• CPC Class Codes: C12N 2502/1347 Smooth muscle cells
• CPC Class Codes: C12N 2502/256 Renal cells
• CPC Class Codes: C12N 2502/28 Vascular endothelial cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2510/02 Cells for production
• CPC Class Codes: C12N 5/0686 Kidney cells
• CPC Class Codes: C12N 5/0697 Artificial constructs associating cells of different lineages, e.g. tissue equivalents blood vessels C12N5/0691

Abstract

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and/or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

Claims

1. A bioartificial device, comprising a semipermeable surface coated with (i) a layer of nonimmortalized endothelial cells, epithelial cells, or a mixture thereof and (ii) pericyte, vascular smooth muscle or mesangial cells or a mixture thereof,

wherein one side of said semipermeable surface is in fluid communication with a blood inlet and the other side is in fluid communication with a blood outlet.

2. The bioartificial device according to claim 1, which is a reabsorption device and wherein said layer (i) comprises renal tubule cells.

3. The bioartificial device of claim 1, which is a filtration device and wherein said layer (i) comprises renal endothelial cells and pericyte, vascular smooth muscle or mesangial cells or a mixture thereof.

4. The bioartificial filtration device according to claim 1, wherein said pericyte cells (ii) are mesangial cells.

5. The bioartificial filtration device according to claim 1, comprising at least a 1:

1 ratio of (a) endothelial cells to (b) pericyte, vascular smooth muscle or mesangial cells or a mixture thereof.