Diagnosing predisposition to fat deposition and associated conditions
First Claim
1. A method for diagnosing a predisposition to fat deposition in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with fat deposition at a polymorphic site in a PLA2G1B nucleotide sequence in a nucleic acid sample from a subject, wherein the PLA2G1B nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO;
1;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO;
2;
(c) a nucleotide sequence which encodes a polypeptide that is 90% identical to the amino acid sequence of SEQ ID NO;
2; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic site;
whereby the presence of the polymorphic variation is indicative of a predisposition to fat deposition in the subject.
2 Assignments
0 Petitions
Accused Products
Abstract
Provided herein are methods for prognosing and diagnosing fat deposition and related disorders (e.g., obesity and non-insulin diabetes dependent mellitus (NIDDM)) in a subject, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for reducing fat deposition and related disorders, and therapeutic methods for reducing fat deposition or treating fat deposition related disorders in a subject. These embodiments are based in part upon an analysis of polymorphic variations of the nucleic acid set forth in SEQ ID NO:1.
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Citations
30 Claims
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1. A method for diagnosing a predisposition to fat deposition in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with fat deposition at a polymorphic site in a PLA2G1B nucleotide sequence in a nucleic acid sample from a subject, wherein the PLA2G1B nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO;
2;
(c) a nucleotide sequence which encodes a polypeptide that is 90% identical to the amino acid sequence of SEQ ID NO;
2; and
(d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic site;
whereby the presence of the polymorphic variation is indicative of a predisposition to fat deposition in the subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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9. The method of claim 1, wherein the fat deposition is central fat deposition in the subject.
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10. The method of claim 1, wherein the subject is a human.
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11. A method for diagnosing a predisposition to leanness in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with leanness at a polymorphic site in a PLA2G1B nucleotide sequence in a nucleic acid sample from a subject, wherein the PLA2G1B nucleotide sequence is selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO;
2;
(c) a nucleotide sequence which encodes a polypeptide that is 90% identical to the amino acid sequence of SEQ ID NO;
2; and
(d) a fragment of a nucleotide sequence of (i), (ii), or (iii) comprising the polymorphic site;
whereby the presence of the polymorphic variation is indicative of leanness in the subject. - View Dependent Claims (12, 13, 14, 15)
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16. A method for identifying a polymorphic variation associated with fat deposition proximal to an incident polymorphic variation associated with fat deposition, which comprises:
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identifying a polymorphic variant proximal to the incident polymorphic variant associated with fat deposition, wherein the incident polymorphic variant is in a PLA2G1B nucleotide sequence and the PLA2G1B nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of;
(a) a polynucleotide sequence set forth in SEQ ID NO;
1;
(b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence encoded by a nucleotide sequence set forth as SEQ ID NO;
1;
or(c) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence that is 90% identical to an amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NO;
1; and
determining the presence or absence of an association of the proximal polymorphic variant with fat deposition. - View Dependent Claims (17, 18, 19, 20, 21)
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22. A method for diagnosing a predisposition to non-insulin dependent diabetes mellitus (NIDDM) in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with NIDDM at a polymorphic site in a PLA2G1B nucleotide sequence in a nucleic acid sample from a subject, wherein the PLA2G1B nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of:
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(a) the nucleotide sequence of SEQ ID NO;
1;
(b) a nucleotide sequence which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO;
2;
(c) a nucleotide sequence which encodes a polypeptide that is 90% identical to the amino acid sequence of SEQ ID NO;
2; and
(d) a fragment of a nucleotide sequence of (i), (ii), or (iii) comprising the polymorphic site;
whereby the presence of the polymorphic variation is indicative of a predisposition to NIDDM in the subject. - View Dependent Claims (23, 24)
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25. A method for identifying a polymorphic variation associated with NIDDM proximal to an incident polymorphic variation associated with NIDDM, which comprises:
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identifying a polymorphic variant proximal to the incident polymorphic variant associated with NIDDM, wherein the incident polymorphic variant is in a PLA2G1B nucleotide sequence and the PLA2G1B nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of;
(a) a polynucleotide sequence set forth in SEQ ID NO;
1;
(b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence encoded by a nucleotide sequence set forth as SEQ ID NO;
1;
or(c) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence that is 90% identical to an amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NO;
1; and
determining the presence or absence of an association of the proximal polymorphic variant with NIDDM. - View Dependent Claims (26, 27, 28, 29, 30)
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Specification