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Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate

  • US 20050239090A1
  • Filed: 06/07/2004
  • Published: 10/27/2005
  • Est. Priority Date: 11/22/1996
  • Status: Active Grant
First Claim
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1. A method of inhibiting or preventing pyrophosphorolysis during synthesis of a nucleic acid molecule, said method comprising (a) combining one or more nucleotides and a nucleic acid template;

  • and (b) incubating the one or more nucleotides and nucleic acid template together with a polymerase and an enzyme selected from the group consisting of a pentosyltransferase, a phosphotransferase with alcohol group as acceptor, a nucleotidyltransferase, and a carboxy-lyase, under conditions sufficient to form a second nucleic acid molecule complementary to all or a portion of the nucleic acid template.

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