Device and method of use for detection and characterization of microorganisms and microparticles
First Claim
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1. An apparatus for capturing a microorganism or microparticle comprising:
- a solid support comprising a surface adapted for use in a scanning probe microscope;
a material deposited in a plurality of discrete domains on the surface, wherein the material is capable of interacting with the microorganism or the microparticle, and wherein exposure of the apparatus to a sample which can contain the microorganism or the microparticle causes the microorganism or microparticle to interact with the material.
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Abstract
The present invention includes a method and apparatus for the detection of a microorganism or microparticle.
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Citations
57 Claims
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1. An apparatus for capturing a microorganism or microparticle comprising:
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a solid support comprising a surface adapted for use in a scanning probe microscope;
a material deposited in a plurality of discrete domains on the surface, wherein the material is capable of interacting with the microorganism or the microparticle, and wherein exposure of the apparatus to a sample which can contain the microorganism or the microparticle causes the microorganism or microparticle to interact with the material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 46)
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15. A method for detecting a microorganism or microparticle in a sample comprising:
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providing a substrate with a surface;
depositing a material on the surface in a plurality of discrete domains, wherein the material is capable of interacting with the microorganism or microparticle;
exposing the material to the sample; and
detecting an interaction between the material and the microorganism by imaging the interaction. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. A method for amplifying a nucleic acid within a microorganism comprising:
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(a) providing a substrate with a surface;
(b) depositing a material on the surface, wherein the material has a capacity to interact with the microorganism;
(c) exposing the material to a sample which can contain the microorganism, wherein the microorganism comprises a nucleic acid, and wherein the microorganism interacts with the material; and
(d) amplifying the nucleic acid of step c in a polymerase chain reaction. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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47. A method for screening antibodies capable of capturing particulate antigens comprising:
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providing a substrate with a surface;
depositing an antibody onto the surface;
exposing the antibody to a component comprising a particulate antigen; and
measuring the interaction between the antibody and the particulate antigen using scanning force microscopy. - View Dependent Claims (48)
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49. A method of screening for an antiviral agent comprising:
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providing a substrate with a surface;
depositing a capture reagent onto the surface;
exposing the surface to sample which can contain a virus and an antiviral agent; and
detecting the virus that interacts with the capture reagent using scanning probe microscopy.
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50. A method of screening for an antiviral agent comprising:
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(a) providing a probe suitable for use in atomic force microscopy;
(b) attaching a virus to the probe;
(c) contacting the probe with a surface, wherein the surface comprises a material that can interact with the virus;
(d) contacting the receptor and the virus of step (c) with a sample that can contain an antiviral agent; and
(e) detecting a change in the interaction between the virus and the receptor, wherein the change is correlated with the presence of an antiviral agent.
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51. A method for detecting the presence of an antibody from an animal comprising:
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providing a substrate with a surface;
depositing the antibody on the surface, wherein the antibody is derived from the animal, and wherein the antibody is capable of interacting with a microorganism;
exposing the antibody to the microorganism; and
detecting an interaction between the antibody and the microorganism. - View Dependent Claims (52, 53)
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54. A method for detecting the presence of a microorganism from an animal comprising:
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providing a substrate with a surface;
depositing an antibody on the surface, wherein the antibody is derived from the animal, and wherein the antibody is capable of interacting with the microorganism;
exposing the antibody to the microorganism; and
detecting an interaction between the antibody and the microorganism. - View Dependent Claims (55)
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56. An apparatus for detecting a microorganism or microparticle comprising:
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a solid support comprising a surface adapted for use in diffraction assay;
a material deposited in a plurality of linear arrays on the surface, wherein the material is capable of interacting with the microorganism or the microparticle, and wherein exposure of the apparatus to a sample which can contain the microorganism or the microparticle causes the microorganism or microparticle to interact with the material. - View Dependent Claims (57)
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Specification