Biosensor

• US 20050239194A1
• Filed: 03/01/2005
• Published: 10/27/2005
• Est. Priority Date: 03/02/2004
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Takahashi, Koji, Higashihara, Akihisa
• Granted Patent: US 7,622,026 B2
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

There is provided a biosensor in which a spacer 6 having a cutout portion is bonded between at least first support 1 and a second support 8 to form a specimen supply path 7 through which a supplied specimen is sucked into a space between the supports 1 and 8, a reagent that reacts with a component in the specimen is placed in the specimen supply path 7, and an air hole 9 for promoting suction of the specimen is formed in the second support; wherein the air hole 9 is shaped such that a diameter y of an opening 91b at one surface of the second support 8 which faces the specimen supply path 7 is larger than a diameter x of an opening 91a at the other surface of the second support, and projecting parts 92a and 92b are formed at the peripheries of the openings 91a and 91b, respectively. Thereby, a sufficient amount of specimen can be sucked regardless of the viscosity of the specimen supplied to the biosensor, and further, the specimen is prevented from flowing over the air hole 9.

Claims

1. A biosensor in which at least first and second supports are bonded together to make a specimen supply path through which a supplied specimen is sucked into a space between the supports, a reagent which reacts with a component of the specimen is disposed in the specimen supply path, and an air hole which leads from the specimen supply path to the outside is formed in the second support,

wherein a diameter of an opening of the air hole at one surface of the second support which faces the specimen supply path is larger than a diameter of an opening of the air hole at the other surface of the second support.

2. A biosensor as defined in claim 1 wherein

the area of the opening at the one surface of the second support facing the specimen supply path is 1.76×

10<sup>−

2</sup>˜

3.14×

10<sup>−

2</sup> mm², and

the area of the opening at the other surface of the second support is 1.96×

10<sup>−

3</sup>˜

7.85×

10<sup>−

3</sup> mm².

3. A biosensor as defined in claim 1 wherein

the openings of the air hole are nearly circular in shape, and

the diameter of the opening at the one surface of the second support facing the specimen supply path is 0.15˜

0.20 mm, and the diameter of the opening at the other surface of the second support is 0.05˜

0.10 mm.

4. A biosensor as defined in claim 1 wherein

said air hole has a first projecting part that is formed on the periphery of the opening at the other surface of the second support, and a second projecting part that is formed on the periphery of the opening at the one surface of the second support facing the specimen supply path.

5. A biosensor as defined in claim 4 wherein

when the thickness of the second support is 0.01 mm˜

0.5 mm and the area of the opening at the other surface of the second support is 1.96×

10<sup>−

3</sup>˜

7.85×

10<sup>−

3</sup> mm², the heights of the first and second projecting parts are 0.005˜

0.04 mm.

6. A biosensor as defined in claim 4 wherein

the height of the first projecting part is larger than the height of the second projecting part.

7. A biosensor as defined in claim 1 wherein

said air hole is formed by perforating the second support with laser irradiation.

8. A biosensor as defined in claim 1 wherein

the shape of the opening of the air hole is any of circle, ellipse, line having a very small width, triangle, square, rectangle, and polygon.