Devices for culturing anaerobic microorganisms and methods of using the same
First Claim
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1. A device for growing microorganisms, comprising:
- a) a supporting substrate having opposing inner and outer surfaces;
b) a cover sheet having opposing inner and outer surfaces affixed to at least one edge of the inner surface of said supporting substrate and positioned to cover at least a portion of said supporting substrate; and
c) media for supporting the growth of microorganisms;
wherein said device comprises an oxygen scavenger.
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Abstract
Culture devices for promoting the growth of microorganisms, especially anaerobic microorganisms, are disclosed. The culture devices include an oxygen scavenging material. In one aspect, the culture device includes a supporting substrate and a cover sheet affixed to at least one edge of the supporting substrate. At least one of the supporting substrate and cover sheet includes the oxygen scavenging material. In another aspect, the culture device is an article that includes a container, e.g. a pouch, including a film having an oxygen scavenging material, with a culture media placed within the container.
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Citations
27 Claims
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1. A device for growing microorganisms, comprising:
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a) a supporting substrate having opposing inner and outer surfaces;
b) a cover sheet having opposing inner and outer surfaces affixed to at least one edge of the inner surface of said supporting substrate and positioned to cover at least a portion of said supporting substrate; and
c) media for supporting the growth of microorganisms;
wherein said device comprises an oxygen scavenger. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An article for culturing microorganisms, comprising:
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a) a container comprising at least one film layer comprising an oxygen scavenger; and
b) a device for growing microorganisms, the device comprising;
i) a supporting substrate having opposing inner and outer surfaces;
ii) a cover sheet having opposing inner and outer surfaces affixed to at least one edge of the inner surface of said supporting substrate and positioned to cover at least a portion of said supporting substrate; and
iii) culture media for supporting the growth of microorganisms;
wherein the device for growing microorganisms is enclosed by the container. - View Dependent Claims (16, 17, 18, 19, 20, 21)
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22. A method for determining microorganism counts in a device comprising a culture media, wherein the device is enclosed in a discrete container comprising an oxygen scavenger, comprising the steps of:
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a) exposing the container comprising the oxygen scavenger to actinic radiation at a dosage sufficient to trigger the oxygen scavenger;
b) inoculating the culture media of the device with a predetermined volume of aqueous test sample;
c) placing the device in said container;
d) closing the container;
e) incubating the device; and
f) counting the number of microorganism colonies growing on the culture media. - View Dependent Claims (23)
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24. A method for determining microorganism counts, comprising the steps of:
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a) exposing a device to actinic radiation at a dose sufficient to trigger an oxygen scavenger within the device, the device comprising i) a supporting substrate having opposing inner and outer surfaces;
ii) a cover sheet having opposing inner and outer surfaces affixed to at least one edge of the inner surface of said supporting substrate and positioned to cover at least a portion of said supporting substrate; and
iii) culture media for supporting the growth of microorganisms;
b) inoculating the culture media of the device with a predetermined volume of aqueous test sample;
c) incubating the device; and
d) counting the number of microorganism colonies growing on the culture media. - View Dependent Claims (25)
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26. A method of making an anaerobic culture media device useful for growing microorganisms comprising:
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a) providing a supporting substrate having opposing inner and outer surfaces that incorporates a culture media on the inner surface;
b) providing a cover sheet having opposing inner and outer surfaces;
c) coating a layer of the cover sheet with adhesive and uniformly affixing a gelling agent and nutrient medium to the inner surface; and
d) affixing the cover sheet to at least one edge of the inner surface of said supporting substrate;
the cover sheet positioned to cover at least a portion of said supporting substrate;
wherein at least one of the supporting substrate and cover sheet comprises an oxygen scavenger and an oxygen barrier. - View Dependent Claims (27)
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Specification