Methods of donor specific crossmatching

• US 20050244404A1
• Filed: 05/16/2005
• Published: 11/03/2005
• Est. Priority Date: 05/16/2002
• Status: Abandoned Application

• Current Assignee: Absorber AB (CareDx, Inc.)
• Original Assignee: Absorber AB (CareDx, Inc.)
• Inventors: Grufman, Per, Sumitran-Holgersson, Suchitra
• Application Number:
  US11/130,306
• Publication Number:
  US 20050244404A1
• Time in Patent Office: Days
• US Class Current: 424/130.100
• CPC Class Codes: G01N 2333/475 Assays involving growth factors
• CPC Class Codes: G01N 2333/71 for growth factors; for growth regulators
• CPC Class Codes: G01N 2800/24 Immunology or allergic disorders
• CPC Class Codes: G01N 2800/245 Transplantation related diseases, e.g. graft versus host disease
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
• CPC Class Codes: G01N 33/56966 Animal cells

Abstract

The detection of endothelial cell antibodies has been proven clinically important for successful organ transplantation. Disclosed are methods of isolating endothelial cell antibodies for donor-specific crossmatching.

Claims

1. A kit for isolating an endothelial cell precursor cell in a sample comprising a first antibody that specifically binds an endothelial cell precursor cell surface marker and a detection reagent.

2. The kit of claim 1, wherein said endothelial cell precursor cell surface marker is Tie2, HLA II, CD31, CD105, CD151, VEGFR-2, CD133, VEGFR-1, CD68, CD14, CD62E, CD106, CD54, VWF or Ac-LDL.

3. The kit of claim 1, wherein the first antibody is a polyclonal antibody.

4. The kit of claim 1, wherein the first antibody is a monoclonal.

5. The kit of claim 1, wherein the kit further comprises written instructions for using the kit to determine whether a test sample contains an endothelial cell precursor cell.

6. The kit according to claim 1, wherein the kit further comprises a positive control that comprises a polypeptide that comprises an antigenic determinant of an endothelial cell precursor cell surface marker protein, wherein the antigenic determinant binds to said first antibody.

7. The kit according to claim 1, wherein the kit further comprises a negative control that comprises a polypeptide that does not comprises an antigenic determinant of an endothelial cell precursor cell surface marker protein.

8. The kit of claim 1, wherein said sample is blood.

9. The kit of claim 1, wherein said kit further comprises a sample collection means.

10. The kit of claim 9, wherein said sample collection means further comprises an anti-coagulant.

11. The kit of claim 10, wherein said anti-coagulant is heparin.

12. The kit of claim 9, wherein said sample collection means is a CPT vacutainer tube.

13. The kit of claim 1, wherein said detection reagent is a second antibody.

14. The kit of claim 13, where said second antibody specifically binds human immunoglobin.

15. The kit of claim 14, wherein said second antibody specifically binds an IgG or IgM immunoglobin.

16. The kit of claim 13, wherein the second antibody is a polyclonal antibody.

17. The kit of claim 13, wherein the second antibody is a monoclonal antibody.

18. The kit of claim 1, wherein the first antibody is immobilized on a solid support.

19. The kit of claim 18, wherein said solid support is, a particle, a microtiter plate, a cuvette, a tube, or a strip.

20. The kit of claim 19, wherein said particle is a microparticle.

21. The kit of claim 20, wherein said microparticle is a paramagnetic particle.