Method
First Claim
1. ) A method for determining the sequence of a nucleic acid molecule comprising the steps of;
- a) providing a single-stranded form of said nucleic acid molecule;
b) hybridizing a primer to said single stranded form of said nucleic acid molecule to form a template/primer complex;
c) enzymatically extending the primer by the addition of a polymerase and a mixture of at least one nucleotide and at least one labeled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide comprises a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in said mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type of nucleotide added to the primer; and
e) repeating steps c) to d) at least once.
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Accused Products
Abstract
A method for determining the sequence of a nucleic acid molecule includes the steps of: a) providing a single-stranded form of the nucleic acid molecule; b) hybridizing a primer to the single stranded form of the nucleic acid molecule to form a template/primer complex; c) enzymatically extending the primer by the addition of a polymerase and a mixture of at least one nucleotide and at least one labeled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide includes a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in the mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type of nucleotide added to the primer; and e) repeating steps c) to d) at least once.
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Citations
18 Claims
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1. ) A method for determining the sequence of a nucleic acid molecule comprising the steps of;
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a) providing a single-stranded form of said nucleic acid molecule;
b) hybridizing a primer to said single stranded form of said nucleic acid molecule to form a template/primer complex;
c) enzymatically extending the primer by the addition of a polymerase and a mixture of at least one nucleotide and at least one labeled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide comprises a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in said mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type of nucleotide added to the primer; and
e) repeating steps c) to d) at least once. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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- 17. ) A kit comprising, in separate compartments, a mixture of at least one nucleotide and at least one labelled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide comprises a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in said mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%, preferably in the range of 5-20 mole-%, 5-30 mole-%, 5-40 mole-% or 5-50 mole-%, and even more preferably in the range of 10-20 mole-%, 10-30 mole-%, 10-40 mole-% or 10-50 mole-%, and a reducing agent.
Specification