Binding acceleration techniques for the detection of analytes

• US 20050244954A1
• Filed: 03/16/2005
• Published: 11/03/2005
• Est. Priority Date: 06/23/1998
• Status: Abandoned Application

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: Blackburn, Gary F., O'Connor, Stephen D., Meade, Thomas J., Terbrueggen, Robert H., Vielmetter, Jost G., Creager, Stephen E., Irvine, Bruce D., Fraser, Scott, Welch, Thomas W.
• Application Number:
  US11/083,780
• Publication Number:
  US 20050244954A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2537/125 Sandwich assay format
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode

Abstract

The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.

Claims

1. A cartridge comprising:

a) a first chamber comprising a surface comprising an array of capture ligands;

b) a second chamber comprising an absorbent pad;

c) at least one channel connecting said first and said second chambers; and

d) a permeation layer material separating said first and said second chambers.

4. A cartridge of any of claims 1-3 wherein said first chamber further comprises a self-assembled monolayer (SAM).

5. A cartridge of claim 4 wherein said SAM comprises insulators.

6. A cartridge of claim 4 wherein said SAM comprises a mixed monolayer.

7. A cartridge of any of claims 1-3 wherein said first surface is printed circuit board (PCB).

8. A cartridge of claim 7 wherein said PCB is fiberglass.

9. A cartridge of claim 7 wherein said PCB is GETEK™

.

10. A cartridge of any of claims 1-3 wherein said capture ligand is attached to said surface via an attachment linker.

11. A cartridge of claim 10 wherein said attachment linker is an insulator.

12. A cartridge of any of claims 1-3 wherein said capture ligand is a protein.

13. A cartridge of any of claims 1-3 wherein said surface further comprises an array of detection electrodes.

14. A cartridge of any of claims 1-3 wherein said permeation layer material is a membrane.

2. A cartridge comprising:

a) a first chamber comprising;

a. a surface comprising an array of capture ligands; and

b. at least a first electrophoresis electrode;

b) a second chamber comprising at least a second electrophoresis electrode;

c) at least one channel connecting said first and said second chambers; and

d) a permeation layer separating said first and said second chambers.

3. The cartridge of claim 2 wherein said second chamber further comprises an absorbent pad.