Microinjection devices and methods of use

• US 20050246783A1
• Filed: 06/23/2005
• Published: 11/03/2005
• Est. Priority Date: 02/13/2001
• Status: Active Grant

• Current Assignee: Synageva BioPharma Corp (Astrazeneca PLC)
• Original Assignee: Avigenics Inc (Astrazeneca PLC)
• Inventors: Christmann, Leandro
• Granted Patent: US 7,339,090 B2
• Time in Patent Office: Days
• US Class Current: 800/19
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: A61M 37/00 Other apparatus for introducing media into the body for reproduction or fertilisation A61B17/425; apparatus for iontophoresis or cataphoresis A61N1/30; Percutany, i.e. introducing medicines into the body by diffusion through the skin salt baths A61H33/04
• CPC Class Codes: C12M 35/00 Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87
• CPC Class Codes: G02B 21/32 Micromanipulators structurally combined with microscopes

Abstract

The present invention provides for microinjection devices comprising a needle and a viewing instrument wherein the viewing instrument provides magnified viewing of an object to an operator from an angle other than a right angle to the object.

Claims

1. A microinjection device comprising a needle and a viewing instrument wherein the viewing instrument provides magnified viewing of an object from an angle other than a right angle to the object.

2. The device of claim 1 wherein the needle is hollow.

3. The device of claim 1 wherein the needle comprises glass.

4. The device of claim 1 comprising a laser light source.

5. The device of claim 4 wherein the laser light source illuminates the needle.

6. The device of claim 4 wherein the laser light travels down the needle.

7. The device of claim 1 wherein the needle includes a bevel.

8. The device of claim 1 comprising an injector.

9. The device of claim 1 comprising an oscillator.

10. The device of claim 9 wherein the oscillator is effective to impart an oscillation to the needle.

11. The device of claim 10 wherein the oscillation of the needle includes an amplitude of between about 0.001 nm and about 100 μ

m.

12. The device of claim 1 wherein the angle is between about 1°

and about 89°

.

13. The device of claim 1 wherein the angle is between about 10°

and about 70°

.

14. The device of claim 1 wherein the viewing instrument comprises a lens.

15. The device of claim 1 wherein the viewing instrument includes a borescope.

16. A method comprising:

viewing a germinal disc under magnification at an angle to the germinal disc of less than 90°

; and

injecting a nucleic acid component to the germinal disc through a needle.

17. The method of claim 16 wherein the nucleic acid component is a vector.

18. The method of claim 17 wherein the vector is a non-viral vector.

19. The method of claim 16 wherein the nucleic acid component is an artificial chromosome.

20. The method of claim 16 wherein the germinal disc is a chicken germinal disc.

21. The method of claim 16 further comprising delivering the germinal disc to the oviduct of a recipient avian female.

22. The method of claim 16 comprising inserting the needle into the germinal disc.

23. The method of claim 16 wherein inserting the needle into the germinal disc comprises penetrating a vitelline membrane.

24. The method of claim 16 comprising allowing the germinal disc to develop into a chick.

25. A method comprising:

viewing the surface of a germinal disc under magnification at an angle to the the germinal disc of less than 90° and

injecting a substance to the germinal disc through a needle.