Incubation method for obtaining solid culture of Zang Zhi, solid culture obtained therefrom, processed products and use thereof

• US 20050246947A1
• Filed: 11/14/2003
• Published: 11/10/2005
• Est. Priority Date: 12/14/2001
• Status: Active Grant

• Current Assignee: Li-Yu Wu, Ming-Huang Lan
• Original Assignee: Li-Yu Wu, Ming-Huang Lan
• Inventors: Lan, Chang-Chou, Lan, Chung-Liang, Lan, Chung-Min, Lan, Ming-Huang
• Granted Patent: US 7,114,287 B2
• Time in Patent Office: Days
• US Class Current: 47/1.100
• CPC Class Codes: A01G 18/20 Culture media, e.g. compost
• CPC Class Codes: A01G 18/40 Cultivation of spawn
• CPC Class Codes: A01G 18/50 Inoculation of spawn
• CPC Class Codes: A01G 18/66 Cultivation bags
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A61K 36/07 Basidiomycota, e.g. Cryptococcus
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: Y10S 435/915 Aspergillus flavus

Abstract

The present invention provides an incubation method for obtaining solid culture of Zang Zhi (Antrodia camphorate) comprising the steps of: incubating a stock strain of Zang Zhi in a PP bag for growth of mycelia, followed by an incubation in the air for fruiting the Zang Zhi body. The invention also provides the solid culture of Zang Zhi obtained by the incubation method, and the products processed therefrom and the use thereof.

Claims

1-49. -49. (canceled)

50. A method for culturing Antrodia camphorata comprising:

(a) culturing spawn of a wild-type fungus of Antrodia campholaita in a bag containing a medium comprising 10-70 wt % of a cellulolytic substance, 10-30 wt % of a starch, 5-15 wt % of a millet, 1-10 wt % of a saccharide, 0.5-2 wt % of a phosphate and 0.1-1 wt % of a sulfite salt at a relative humidity of 60-80 wt %, the medium having a neutral pH, the culturing being carried out at a temperature of 5-32°

C. in an atmosphere comprising 0.1-1 vol % carbon dioxide for a period sufficient to grow mycelium from the spawn;

(b) removing the medium from the bag;

(c) exposing the removed medium to air and maintaining the removed medium in the air for a period of days sufficient to form a solid fruiting body, said removed medium being maintained in the air at a temperature which differs between days and nights with the removed medium being maintained during the days at a temperature between 20° and

30°

C. and during the nights at a temperature of 10-12°

C., wherein the humidity of the air is between 90-95% and the air contains less than 1 vol % of carbon dioxide; and

(d) recovering the solid fruiting body.

51. The method according to claim 50, wherein the medium removed in step (c) is maintained during the days at a temperature that is 15°

C. higher than the temperature during the nights.

52. The method according to claim 50, wherein the solid fruiting body is of conoid shape and is about 10-30 cm in diameter, 15-30 cm in height and 0.2-0.6 kg in weight.

53. The method according to claim 50, wherein the cellulolytic substance is mushroom.

54. The method according to claim 53, wherein the starch is potato.

55. The method according to claim 54, wherein the millet is rice bran.

56. The method according to claim 50, wherein the culturing in step (a) is carried out for a period of 50-80 days.

57. The method according to claim 56, wherein the removed medium is maintained in the air for the period of 20-50 days.

58. The method according to claim 50, wherein the bag comprises 65 wt % of stems, stalks or fruits of a grass plant or cellulolytic spelk, 20 wt % of potato, 10 wt % of rice bran, 3.5 wt % of glucose, 1 wt % of potassium phosphate and 0.5 wt % of magnesium sulfate.

59. The method according to claim 50, wherein the culturing in step (a) is carried out at a temperature of 5°

C. to 28°

C.

60. The method according to claim 51, wherein the culturing in step (a) is carried out at a temperature of 28°

C.

61. The method according to claim 50, wherein the culturing in step (a) is carried out at a humidity of 60-80%.

62. The method according to claim 50, wherein the removed medium is maintained in humid air having a humidity of 90-95%.

63. The method according to claim 62, wherein the humid air has less than 1% of carbon dioxide.

64. The method according to claim 50 comprising, prior to step (a), the step of multiplying the spawn.

65. The method according to claim 64, wherein the spawn are multiplied by a liquid culture fermentation.

66. The method according to claim 50, further comprising grinding the solid fruiting body recovered in step (d) to form small pieces and then homogenizing the small pieces with water or alcohol to form a concentrated liquid.

67. The method according to claim 66, wherein the concentrated liquid is dehydrated to form a dry powder.

68. The method according to claim 67, wherein the powder is wetted and then dried to form granules.

69. The method according to claim 50, wherein step (c) is carried out in moving air.