Process for immobilization of protein catalysts, product, and use

• US 20050250193A1
• Filed: 05/07/2004
• Published: 11/10/2005
• Est. Priority Date: 05/07/2004
• Status: Active Grant

• Current Assignee: GM Global Technology Operations LLC (General Motors Company)
• Original Assignee: GM Global Technology Operations Incorporated (Motors Liquidation Co. GUC Trust)
• Inventors: Johnson, John T., Dziedzic, Daniel, Gross, Kenneth B.
• Granted Patent: US 7,642,076 B2
• Time in Patent Office: Days
• US Class Current: 435/174
• CPC Class Codes: C12N 11/06 attached to the carrier via a bridging agent
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: Y10T 428/31507 Of polycarbonate
• CPC Class Codes: Y10T 428/31511 Of epoxy ether
• CPC Class Codes: Y10T 428/31551 Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31725 Of polyamide
• CPC Class Codes: Y10T 428/31786 Of polyester [e.g., alkyd, etc.]
• CPC Class Codes: Y10T 428/31931 Polyene monomer-containing
• CPC Class Codes: Y10T 428/31938 Polymer of monoethylenically unsaturated hydrocarbon

Abstract

An immobilized protein catalyst is prepared by applying an adhesive to a polymeric support, applying a layer of a globular protein over the layer of adhesive, binding a crosslinking agent to the protein layer, and binding the protein catalyst by reaction with the crosslinking agent.

Claims

1. A method of preparing an immobilized protein catalyst, comprising steps of

(a) applying an adhesive to a polymeric support;

(b) applying a layer of a globular protein over the layer of adhesive;

(c) binding a crosslinking agent to the protein layer; and

(d) binding a protein catalyst by reaction with the crosslinking agent.

2. A method of preparing an immobilized protein catalyst according to claim 1, comprising applying a one-component, solvent-based adhesive in step (a).

3. A method of preparing an immobilized protein catalyst according to claim 1, comprising applying a crosslinking agent having a plurality of groups reactive with a member selected from the group consisting of amine groups, carboxylic acid groups, phenolic groups, sufhydryl groups, and imidazole groups.

4. A method of preparing an immobilized protein catalyst according to claim 1, comprising applying a crosslinking agent having a plurality of groups reactive with hydrogens of amine groups in step (c).

5. A method of preparing an immobilized protein catalyst according to claim 1, wherein the adhesive applied in step (a) is dried before step (b).

6. A method of preparing an immobilized protein catalyst according to claim 1, wherein the polymeric support comprises a member selected from the group consisting of polyethylene, polypropylene, polybutadiene, polyamide, polycarbonate, vinyl polymers, polypropylene glycol, polyesters, phenolic polymers, epoxy polymers, polyurethanes, acrylic polymers, styrene polymers and copolymers, and combinations thereof.

7. A method of preparing an immobilized protein catalyst according to claim 1, wherein the adhesive is applied as a dilute solution.

8. A method of preparing an immobilized protein catalyst according to claim 7, wherein the adhesive is diluted in organic solvent selected from the group consisting of hexane, heptane, toluene, xylene, methylene chloride, carbon tetrachloride, trichloromethane, Aromatic 100, tetrahydrofuran, acetone, 2-propanol, methanol, acetronitrile and combinations thereof and wherein the adhesive is applied in step (a) by dipping the support into the adhesive diluted in organic solvent.

9. A method of preparing an immobilized protein catalyst according to claim 1, wherein the globular protein comprises an albumin.

10. A method of preparing an immobilized protein catalyst according to claim 1, wherein the globular protein comprises ovalbumin.

11. A method of preparing an immobilized protein catalyst according to claim 1, wherein the crosslinking agent has a plurality of aldehyde groups, isocyanate groups, oxirane groups, thio groups, sulfhydryl groups, amine groups, or photoreactive groups.

12. A method of preparing an immobilized protein catalyst according to claim 1, wherein the crosslinking agent comprises glutaraldehyde.

13. A method of preparing an immobilized protein catalyst according to claim 1, wherein the protein catalyst comprises carbonic anhydrase.

14. An immobilized protein catalyst, comprising

a polymeric support,

an adhesive layer over the polymeric support,

a layer of a globular protein over the adhesive layer, and

a protein catalyst reacted onto the layer of globular protein.

15. An immobilized protein catalyst according to claim 14, wherein the polymeric support comprises a member selected from the group consisting of polyethylene, polypropylene, polybutadiene, polyamide, polycarbonate, vinyl polymers, polypropylene glycol, polyesters, phenolic polymers, epoxy polymers, polyurethanes, acrylic polymers, styrene polymers and copolymers, and combinations thereof.

16. An immobilized protein catalyst according to claim 14, wherein the globular protein comprises an albumin.

17. An immobilized protein catalyst according to claim 14, wherein the globular protein comprises ovalbumin.

18. An immobilized protein catalyst according to claim 14, wherein the protein catalyst is reacted onto the layer of globular protein by glutaraldehyde.

19. An immobilized protein catalyst according to claim 14, wherein the protein catalyst comprises carbonic anhydrase.

20. A method of hydrating carbon dioxide, comprising bringing carbon dioxide into contact with water in the presence of the immobilized carbonic anhydrase according to claim 19.