Drug discovery assays based on the biology of chronic disease
First Claim
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1. A method for evaluating the ability of a compound to affect expression of a gene natural to a cell, the method comprising the steps of:
- a. selecting a transcription complex natural to the cell, wherein the transcription complex is limiting;
b. selecting a polynucleotide foreign to the cell, wherein the foreign polynucleotide can bind the transcription complex;
c. selecting a compound of interest;
d. combining the compound with a system, wherein the system includes a known copy number of the foreign polynucleotide;
e. assaying the copy number of the foreign polynucleotide in the system after the combination; and
f. identifying whether the compound can modify the copy number.
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Abstract
Using the recently discovered biology of chronic disease, the invention presents new methods for evaluating the effectiveness of a compound for use in modulating the progression of chronic disease, for determining whether a subject has a chronic disease, or has an increased risk of developing clinical symptoms associated with such disease, and for treating chronic disease.
47 Citations
20 Claims
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1. A method for evaluating the ability of a compound to affect expression of a gene natural to a cell, the method comprising the steps of:
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a. selecting a transcription complex natural to the cell, wherein the transcription complex is limiting;
b. selecting a polynucleotide foreign to the cell, wherein the foreign polynucleotide can bind the transcription complex;
c. selecting a compound of interest;
d. combining the compound with a system, wherein the system includes a known copy number of the foreign polynucleotide;
e. assaying the copy number of the foreign polynucleotide in the system after the combination; and
f. identifying whether the compound can modify the copy number. - View Dependent Claims (2, 3, 4)
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5. A method for evaluating an effectiveness of a compound for use in modulating progression of a disease, the method comprising the steps of:
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a. selecting a transcription complex natural to a cell, wherein the transcription complex is limiting;
b. selecting a polynucleotide foreign to the cell, wherein the polynucleotide can bind the transcription complex;
c. selecting a compound of interest;
d. combining the compound with a system, wherein the system includes a known copy number of the foreign polynucleotide;
e. assaying the copy number of the foreign polynucleotide in the system after the combination; and
f. identifying whether the compound can modify the copy number. - View Dependent Claims (6, 7, 8, 9)
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10. A method for evaluating the ability of a compound to affect expression of a gene natural to a cell, the method comprising the steps of:
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a. selecting a transcription complex natural to the cell, wherein the transcription complex is limiting;
b. selecting a polynucleotide foreign to the cell, wherein the foreign polynucleotide can bind the transcription complex;
c. selecting a compound of interest;
d. combining the compound with a system, wherein the system includes the transcription complex and the foreign polynucleotide;
e. assaying the complex between the transcription complex and the foreign polynucleotide in the system after the combination; and
f. identifying whether the compound can modify the complex between the transcription complex and the foreign polynucleotide. - View Dependent Claims (11, 12, 13, 14)
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15. A method for evaluating an effectiveness of a compound for use in modulating progression of a disease, the method comprising the steps of:
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a. selecting a transcription complex natural to a cell, wherein the transcription complex is limiting;
b. selecting a polynucleotide foreign to the cell, wherein the foreign polynucleotide can bind the transcription complex;
c. selecting a compound of interest;
d. combining the compound with a system, wherein the system includes the transcription complex;
e. assaying the transcription complex in the system after the combination; and
f. identifying whether the compound can modify the transcription complex. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification