Anti-tag antibody chip for protein interaction analysis

• US 20050255526A1
• Filed: 05/04/2005
• Published: 11/17/2005
• Est. Priority Date: 05/12/2004
• Status: Abandoned Application

• Current Assignee: Hitachi High-Technologies Corporation (Hitachi, Ltd.)
• Original Assignee: Hitachi High-Technologies Corporation (Hitachi, Ltd.)
• Inventors: Kanda, Katsuhiro, Sasakura, Yukie
• Application Number:
  US11/121,118
• Publication Number:
  US 20050255526A1
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

It is an object of the present invention to conduct function analysis of proteins arranged with high density on a solid phase in a high-throughput manner, regardless of protein varieties. The present invention is characterized in that it immobilizes target proteins on a substrate while maintaining the structures and the functions thereof, and that it carries out interaction analysis of such proteins. For example, target proteins are prepared in a state where one end of each protein is fused to a peptide as a tag or to a polypeptide by genetic engineering methods. Then, the target proteins are bonded to the substrate via a layer formed thereon, comprising anti-tag antibodies. The target proteins are arrayed while they are maintained in a liberated state on a solid phase, and the interaction analysis is carried out. According to the present invention, the interaction analysis of target proteins can be carried out without affecting the unstable structures and the functions thereof. For example, the present invention can be applied as a platform for interaction analysis, screening, quantitation, expression profiling, or the like, regarding proteins.

Claims

1. An anti-tag antibody chip comprising a layer including an anti-tag antibody formed on a substrate.

2. A method for manufacturing an anti-tag antibody chip, comprising washing and drying a substrate for an anti-tag antibody chip, processing the washed substrate with an aminosilane compound, processing the aminosilane substrate with a protein immobilizing reagent, and processing the substrate coated with the protein immobilizing reagent with an anti-tag antibody.

3. The method for manufacturing an anti-tag antibody chip according to claim 2, wherein the aminosilane compound comprises 3-aminopropyltriethoxysilane and the protein immobilizing reagent comprises calixarene.

4. The method for manufacturing an anti-tag antibody chip according to claim 2, comprising, after the substrate coated with the protein immobilizing reagent is processed with the anti-tag antibody, blocking a portion of the surface of the substrate where the anti-tag antibody is not immobilized using a blocking agent.

5. A tag-fusion target protein array comprising a tag-fusion target protein immobilized on a substrate via a layer including an anti-tag antibody.

6. The tag-fusion target protein array according to claim 5, wherein the tag-fusion target protein comprises a peptide or a polypeptide in the form of a tag added to an end of either an N-terminal or a C-terminal thereof or within the amino acid sequence of a target protein, depending on necessity, via a genetic engineering method.

7. A method for manufacturing a tag-fusion target protein array, comprising manufacturing an anti-tag antibody chip, and immobilizing a tag-fusion target protein on the anti-tag antibody chip via a layer including an anti-tag antibody.

8. The method for manufacturing a tag-fusion target protein array according to claim 7, wherein the tag-fusion target protein comprises a peptide or a polypeptide in the form of a tag added to an end of either an N-terminal or a C-terminal thereof or within the amino acid sequence of a target protein, depending on necessity, via a genetic engineering method.

9. A method for analyzing a protein interaction, comprising causing a solution including an interaction substance to react with a tag-fusion target protein array, wherein a tag-fusion target protein is arrayed on a substrate via a layer including an anti-tag antibody.

10. The method for analyzing a protein interaction according to claim 9, wherein the tag-fusion target protein comprises a peptide or a polypeptide in the form of a tag added to an end of either an N-terminal or a C-terminal thereof or within the amino acid sequence of a target protein, depending on necessity, via a genetic engineering method.

11. A platform for protein interaction analysis, comprising a tag-fusion target protein arrayed on an anti-tag antibody chip.

12. A method for purifying a tag-fusion target protein, comprising directly subjecting a product before purification expressed in a genetic engineering manner to an anti-tag antibody chip, and washing and removing debris in a state where only a tag-fusion target protein is bonded to an anti-tag antibody.

13. A protein interaction analysis apparatus comprising an anti-tag antibody chip mount mechanism for mounting an anti-tag antibody chip and moving thereof, a dispensing mechanism for dispensing a sample including a tag-fusion target protein to the anti-tag antibody chip, a position recognition mechanism capable of dispensing to the same spot on the chip in a repeated manner, and a reading mechanism of a labeled sample on the anti-tag antibody chip after reaction.

14. The protein interaction analysis apparatus according to claim 13, wherein the labeled sample comprises a fluorescence-labeled sample and the reading mechanism comprises a fluorescence scanner or a two-photon excitation scanner.

15. The protein interaction analysis apparatus according to claim 13, wherein the labeled sample comprises a non-fluorescence-labeled sample and the reading mechanism comprises a surface plasmon resonance (SPR) apparatus or an optical waveguide detection apparatus.