Comprehensive food allergy test
First Claim
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1. A kit for determining the presence of allergen-specific immunoglobulins and immuncomplex C3b (IC-C3b) in a biological sample comprising:
- (a) a solid support comprising an immobilized allergen that is to be exposed to a biological sample thereby binding and immobilizing allergen-specific immunoglobulins and immuncomplex C3b; and
(b) at least two labeled binding partners;
(i) a first labeled binding partner that specifically binds the immobilized allergen-specific immunoglobulins; and
(ii) a second labeled binding partner that specifically binds the immobilized immunocomplex C3b.
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Abstract
The present invention relates to methods and devices for detecting biological entities and components associated with hypersensitivity reactions in patients with food allergies. Specifically, the assays of the invention are capable of qualitatively and/or quantitatively measuring IgG, IgA, IgM and/or IgE antibodies and immunocomplexed C3b (IC-C3b) produced as a result of exposure to food.
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Citations
19 Claims
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1. A kit for determining the presence of allergen-specific immunoglobulins and immuncomplex C3b (IC-C3b) in a biological sample comprising:
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(a) a solid support comprising an immobilized allergen that is to be exposed to a biological sample thereby binding and immobilizing allergen-specific immunoglobulins and immuncomplex C3b; and
(b) at least two labeled binding partners;
(i) a first labeled binding partner that specifically binds the immobilized allergen-specific immunoglobulins; and
(ii) a second labeled binding partner that specifically binds the immobilized immunocomplex C3b. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of determining the presence of allergen-specific immunoglobulins and immuncomplex C3b (IC-C3b) in a biological sample comprising:
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(a) exposing a solid support comprising an immobilized allergen to a biological sample;
(b) washing unbound molecules from the biological samples from the solid support;
(c) exposing said solid support to at least two labeled binding partners;
(i) a first labeled binding partner that specifically binds the immobilized allergen-specific immunoglobulins; and
(ii) a second labeled binding partner that specifically binds the immobilized immuncomplex C3b;
(d) washing unbound labeled binding partners from the solid support;
(e) detecting the presence of label bound to the solid support; and
correlating it with the presence of allergen specific IC-C3b and allergen specific immunoglobulins in the biological sample. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification