Capillary multi-channel optical flow cell

• US 20050257885A1
• Filed: 04/08/2005
• Published: 11/24/2005
• Est. Priority Date: 05/24/2004
• Status: Abandoned Application

• Current Assignee: Nanostream, Inc.
• Original Assignee: Nanostream, Inc.
• Inventors: Hobbs, Steven E.
• Application Number:
  US11/102,907
• Publication Number:
  US 20050257885A1
• Time in Patent Office: Days
• US Class Current: 156/293
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/056 Laminated construction
• CPC Class Codes: G01N 2030/746 detecting along the line of flow, e.g. axial
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 30/466 with separation columns in parallel
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J

Abstract

A first multi-channel optical flow cell includes a two end blocks disposed around a channel-defining flow layer, with a first end block having multiple inlet ports each containing an associated optical fiber and fluid conduit terminated substantially flush against an inner surface of the first end block. The second end block may have multiple outlet ports each containing at least one of an additional optical fiber and additional fluid conduit. A method for fabricating a multi-channel flow cell includes inserting a first plurality of optical fibers and a first plurality of fluid conduits through a plurality of inlet ports defined in a first end block, sealing the optical fibers and conduits, polishing the optical fibers, and then positioning and joining a channel-defining flow layer between the first end block and a second end block.

Claims

1. A method for fabricating a multi-channel flow cell, the method comprising the steps of:

providing a first end block having a first inner surface and defining a plurality of inlet ports;

providing a second end block having a second inner surface and defining a plurality of outlet ports;

providing a first flow layer defining a first plurality of flow channels and having a first thickness;

inserting a first plurality of optical fibers through the plurality of inlet ports;

inserting a first plurality of fluid conduits through the plurality of inlet ports;

sealing the first plurality of optical fibers and the first plurality of fluid conduits;

polishing the first plurality of optical fibers;

positioning the first flow layer between the first inner surface and the second inner surface; and

directly or indirectly joining the first flow layer, the first end block, and the second end block.

2. The method of claim 1 wherein the sealing step includes potting with a sealant.

3. The method of claim 1 wherein any inlet port of the plurality of inlet ports contains both an optical fiber of the first plurality of optical fibers and a fluid conduit of the first plurality of fluid conduits.

4. The method of claim 1, further comprising the step of trimming the first plurality of optical fibers substantially flush with the first inner surface.

5. The method of claim 1 wherein the polishing step is performed by polishing all of the optical fibers of the first plurality of optical fibers substantially simultaneously.

6. The method of claim 1, further comprising the steps of:

inserting a second plurality of optical fibers into the plurality of outlet ports;

inserting a second plurality of fluid conduits into the plurality of outlet ports;

sealing the second plurality of optical fibers and the second plurality of fluid conduits; and

polishing the second plurality of optical fibers.

7. The method of claim 6 wherein any outlet port of the plurality of outlet ports contains both an optical fiber of the second plurality of optical fibers and a fluid conduit of the second plurality of fluid conduits.

8. The method of claim 6, further comprising the step of trimming the second plurality of optical fibers substantially flush with the second inner surface.

9. The method of claim 6 wherein the polishing step is performed by polishing all of the optical fibers of the second plurality of optical fibers substantially simultaneously.

10. The method of claim 1, further comprising the steps of:

inserting a second plurality of fluid conduits into the plurality of outlet ports;

sealing the second plurality of fluid conduits; and

trimming the second plurality of fluid conduits substantially flush with the second inner surface.

11. The method of claim 1, further comprising the steps of:

providing a second flow layer having a second thickness;

separating the first flow layer, the first end block, and the second end block;

positioning the second flow layer between the first inner surface and second inner surface; and

joining the second flow layer, the first end block, and the second end block.

13. The method of claim 1, further comprising the steps of:

providing a first gasket defining a first plurality of orifices; and

positioning the first gasket between the first inner surface and the first flow layer.

14. The method of claim 13, further comprising the steps of:

providing a second gasket defining a second plurality of orifices; and

positioning the second gasket between the second inner surface and the first flow layer.

12. The method of 11 wherein the first thickness differs from the second thickness.

15. A multi-channel optical flow cell comprising:

a first end block having a first inner surface and defining a plurality of inlet ports;

a flow layer having a first outer surface, having a second outer surface, and defining a plurality of flow channels;

a second end block having a second inner surface and defining a plurality of outlet ports;

a first plurality of optical fibers; and

a first plurality of fluid conduits;

wherein each optical fiber of the first plurality of optical fibers is terminated substantially flush with the first inner surface and is affixed within a different inlet port of the plurality of inlet ports;

wherein each fluid conduit of the first plurality of fluid conduits is terminated substantially flush with the first inner surface and is affixed within a different inlet port of the plurality of inlet ports;

wherein the flow layer is disposed between the first end block and the second end block;

wherein each fluid conduit of the first plurality of fluid conduits is in fluid communication with a different flow channel of the plurality of flow channels; and

wherein each optical fiber of the first plurality of optical fibers is in optical communication with a different flow channel of the plurality of flow channels.

16. The flow cell of claim 15, further comprising:

a second plurality of optical fibers; and

a second plurality of fluid conduits;

wherein each optical fiber of the second plurality of optical fibers is terminated substantially flush with the second inner surface and is affixed within a different outlet port of the plurality of outlet ports;

wherein each fluid conduit of the second plurality of fluid conduits is terminated substantially flush with the second inner surface and is affixed within a different outlet port of the plurality of outlet ports;

wherein each fluid conduit of the second plurality of fluid conduits is in fluid communication with a different flow channel of the plurality of flow channels; and

wherein each optical fiber of the second plurality of optical fibers is in optical communication with a different flow channel of the plurality of flow channels.

17. The flow cell of claim 15, further comprising:

a second plurality of fluid conduits;

wherein each fluid conduit of the second plurality of fluid conduits is terminated substantially flush with the second inner surface and is affixed within a different outlet port of the plurality of outlet ports; and

wherein each fluid conduit of the second plurality of fluid conduits is in fluid communication with a different flow channel of the plurality of flow channels.

18. The flow cell of claim 15, further comprising a first gasket defining a plurality of orifices disposed between the first inner surface and the first outer surface.

19. The flow cell of claim 15, further comprising a second gasket defining a plurality of orifices disposed between the second inner surface and the second outer surface.

20. The flow cell of claim 15 wherein the flow layer comprises any of:

a fluoropolymer, a perfluropolymer, poly(ether ether ketone), fused silica, sapphire, quartz, polyimide, and stainless steel.

21. The flow cell of claim 15 wherein at least a portion of the flow layer is substantially optically transmissive.

22. The flow cell of claim 15 wherein at least a portion of the flow layer transmits at least about eighty percent of radiation wavelengths between about 200 nanometers and about 2000 nanometers.

23. The flow cell of claim 15 wherein at least a portion of the flow layer has a refractive index less than or equal to about 1.3.

24. A high-throughput analytical system comprising:

the flow cell of claim 15;

at least one radiation source in optical communication with the plurality of flow channels; and

a multi-channel detector having a plurality of sensors in optical communication with the plurality of flow channels.

25. The system of claim 24 wherein at least a portion of each flow channel of the plurality of flow channels is optically imaged with a different sensor of the plurality of sensors.

26. The system of claim 24, further comprising a plurality of analytical process regions adapted to perform a plurality of substantially concurrent analytical processes, wherein each flow channel of the plurality of flow channels is in fluid communication with a different analytical process region of the plurality of analytical process regions.

27. The system of claim 26 wherein the plurality of analytical processes comprises chemical or biochemical separation processes.

28. The system of claim 27 wherein the chemical or biochemical separation processes comprise any of:

chromatographic, electrophoretic, electrochromatographic, immunoaffinity, gel filtration, and density gradient separations.

29. The system of claim 24 wherein the at least one radiation source comprises a plurality of radiation sources, the system further comprising a radiation source selection element.

30. The system of claim 24 wherein the multi-channel detector comprises any of:

a multi-channel photomultiplier, a multi-channel charge-coupled device, and a photodiode array.

31. The system of claim 24 wherein the multi-channel detector measures absorbance.

32. The system of claim 24 wherein the multi-channel detector measures fluorescence.

33. A multi-channel optical flow cell comprising:

a first end block defining a plurality of inlet ports and a plurality of outlet ports;

a flow layer defining a plurality of flow channels, with each flow channel of the plurality of flow channels being in fluid communication with a different inlet port of the plurality of inlet ports and being in fluid communication with a different outlet port of the plurality of outlet ports;

a second end block;

a first plurality of optical fibers;

a second plurality of optical fibers;

a first plurality of fluid conduits; and

a second plurality of fluid conduits;

wherein;

each flow channel of the plurality of flow channels is in optical communication with at least one optical fiber of the first plurality of optical fibers and with at least one optical fiber of the second plurality of optical fibers;

each fluid conduit of the first plurality of fluid conduits is affixed within a different inlet port of the plurality of inlet ports;

each fluid conduit of the second plurality of fluid conduits is affixed within a different outlet port of the plurality of outlet ports;

the flow layer is disposed between the first end block and the second end block.

34. The flow cell of claim 33, further comprising:

a first optical fiber termination block having a first surface, wherein each optical fiber of the first plurality of optical fibers is terminated substantially flush with the first surface; and

a second optical fiber termination block having a second surface, wherein each optical fiber of the second plurality of optical fibers is terminated substantially flush with the second surface;

wherein the flow layer is disposed between the first optical fiber termination block and the second optical fiber termination block, with the first optical fiber termination block and second optical fiber termination block being optically coupled through the flow layer.

35. The flow cell of claim 34 wherein:

the flow layer has an opposing third surface and fourth surface, with the first end block being disposed adjacent to the third surface and the second end block being disposed adjacent to the fourth source; and

the flow layer has an opposing fifth surface and sixth surface, with the first optical fiber termination block being disposed adjacent to the fifth surface and the second optical fiber termination block being disposed adjacent to the sixth surface.

36. The flow cell of claim 33 wherein the first end block and the flow layer are integrated into a unitary member.

37. The flow cell of claim 33 wherein any of the second end block and at least a portion of the flow layer comprises a substantially optically transmissive material.