Electrochemical assay device and related methods
First Claim
1. An electrochemical test device comprising a bottom substrate having a first electrode disposed thereon, a top substrate having a second electrode disposed thereon, and a spacer disposed between the top and bottom substrates and having an opening therein, whereby a sample-receiving space is defined that has a bottom surface having the first electrode disposed thereon, a top surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer;
- and a reagent comprising a redox active material which is oxidized at the first electrode and reduced at the second electrode when the device is used;
wherein in the test device prior to introduction of a liquid sample, the reagent is disposed in a layer covering at least a portion of the first or second electrode and at least a portion of the side walls.
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Accused Products
Abstract
An electrochemical test device is provided having a base layer with a first electrode thereon and a top layer with a second electrode thereon. The two electrodes are separated by a spacer layer having an opening therein, such that a sample-receiving space is defined with one electrode on the top surface, the other electrodes on the bottom surface and side walls formed from edges of the opening in the spacer. Reagents for performing the electrochemical reaction are deposited on one of the electrodes and on the side walls of the sample-receiving space.
135 Citations
25 Claims
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1. An electrochemical test device comprising
a bottom substrate having a first electrode disposed thereon, a top substrate having a second electrode disposed thereon, and a spacer disposed between the top and bottom substrates and having an opening therein, whereby a sample-receiving space is defined that has a bottom surface having the first electrode disposed thereon, a top surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer; - and
a reagent comprising a redox active material which is oxidized at the first electrode and reduced at the second electrode when the device is used;
wherein in the test device prior to introduction of a liquid sample, the reagent is disposed in a layer covering at least a portion of the first or second electrode and at least a portion of the side walls. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of making an electrochemical test device comprising the steps of:
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(a) forming a bottom substrate having a first electrode disposed thereon;
(b) forming a spacer layer on the bottom substrate, said spacer layer having an opening formed therein through which the first electrode is exposed and side walls within the opening;
(c) introducing a liquid reagent comprising a redox active material into the opening in the spacer layer, (d) drying the liquid reagent to form a dried reagent, wherein the liquid reagent is introduced to the opening in such a manner that upon drying a dried reagent layer is formed covering at least a portion of the first electrode and at least a portion of the side walls; and
(e) placing a top substrate having a second electrode disposed thereon on the spacer layer aligned to have the second electrode facing the first electrode, thereby forming a sample-receiving space that has a first surface having the first electrode disposed thereon, a second surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification