Biosensor

• US 20050258052A1
• Filed: 05/06/2005
• Published: 11/24/2005
• Est. Priority Date: 09/12/1997
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Therasense Incorporated (Abbott Laboratories Incorporated)
• Inventors: Mure, Hiroki, Shirakawa, Hiroshi, Gotoh, Masao
• Granted Patent: US 7,713,406 B2
• Time in Patent Office: Days
• US Class Current: 205/792
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

Claims

1-47. -47. (canceled)

48. A biosensor comprising:

a first layer having a first length and a second layer having a second length, the first and second layers being fixed together by a third layer having a third length;

a tapered part; and

at least one electrode provided on at least one of the first and second layers.

49. The biosensor of claim 48, wherein the tapered part includes an opening for admitting sample.

50. The biosensor of claim 49, further comprising a space for housing a sample and the opening is in communication with the space for housing the sample.

51. The biosensor of claim 50, wherein the space has a volume that ranges from about 0.5-10 microliters.

52. The biosensor of claim 48, wherein the at least one electrode is a working electrode and a tip part of the working electrode is provided on the tapered part.

53. The biosensor of claim 52, further comprising a counter electrode.

54. The biosensor of claim 53, wherein a tip part of the counter electrode is provided on the tapered part.

55. The biosensor of claim 53, wherein the working electrode and the counter electrode are provided on a same layer.

56. The biosensor of claim 55, wherein the same layer is the first layer and a length of the first layer is greater than a length of the second layer.

57. The biosensor of claim 53, wherein the working electrode and the counter electrode are provided on different layers.

58. The biosensor of claim 53, further comprising a reference electrode and a tip part of the reference electrode is provided on the tapered part.

59. The biosensor of claim 58, wherein the working electrode, counter electrode and reference electrode are provided on a same layer.

60. The biosensor of claim 59, wherein the same layer is the first layer and a length of the first layer is greater than a length of the second layer.

61. The biosensor of claim 48, wherein the third layer comprises adhesive.

62. The biosensor of claim 48, further comprising an analyte-responsive enzyme.

63. The biosensor of claim 62, wherein the analyte-responsive enzyme is selected from the group of glucose oxidase and glucose dehydrogenase.

64. The biosensor of claim 48, wherein the biosensor is connectable to a device body having a control part including a display part, and the biosensor further comprises a biosensor insertion signal terminal for interconnecting, upon connecting the biosensor to the device body, two input terminals of the device body to start the control part of the device body.

65. A biosensor comprising:

an upper plate having a first length,

a lower plate having a second length,

a space formed between the two plates for housing a sample therein,

a layer having a third length for fixing the two plates together,

the space for housing the sample being partially opened at a peripheral part thereof and partially closed by the layer,

a working electrode and a counter electrode fixed relative to the working electrode,

an end having a tapered part and a tip part of the working electrode, and

a tip part of the counter electrode or both tips of the working and counter electrodes being provided on the tapered part.

66. The biosensor of claim 65, further comprising an analyte-responsive enzyme.

67. The biosensor of claim 66, wherein the analyte-responsive enzyme is selected from the group of glucose oxidase and glucose dehydrogenase.

68. The biosensor of claim 65, wherein the biosensor is connectable to a device body having a control part including a display part and the biosensor further comprises a biosensor insertion signal terminal for interconnecting, upon connecting the biosensor to the device body, two input terminals of the device body to start the control part of the device body.

69. The biosensor of claim 65, wherein the tapered part includes an opening for admitting sample.

70. The biosensor of claim 69, further comprising a space for housing a sample and the opening is in communication with the space for housing a sample.

71. The biosensor of claim 65, wherein the working electrode and the counter electrode are provided on a same plate.

72. The biosensor of claim 71, wherein the same plate is the lower plate and a length of the lower plate is greater than a length of the upper plate.

73. A method of measuring a concentration of an analyte in a subject, the method comprising:

contacting body fluid of the subject with a biosensor having at least three layers and a tapered end to generate an electrical signal, the three different layers having different lengths; and

determining from the generated electrical signal a concentration of an analyte in body fluid.

74. The method of measuring a concentration of an analyte in the subject as claimed in claim 73, wherein the body fluid is contacted with the biosensor at the tapered end.

75. The method of measuring a concentration of an analyte in the subject as claimed in claim 74, wherein the tapered end comprises an opening in communication with a space for housing the body fluid, and the method comprises admitting the body fluid into the space through the opening in the tapered end.

76. The method of measuring a concentration of an analyte in the subject as claimed in claim 73, wherein the body fluid ranges from about 0.5 to about 10 microliters.

77. The method of measuring a concentration of an analyte in the subject as claimed in claim 73, wherein the biosensor comprises an analyte-responsive enzyme.