Process and system for crosslinking polynucleotide molecules
First Claim
1. A nucleic acid complex prepared by a process, comprising:
- providing a plurality of first nucleic acids, a plurality of second nucleic acids, and a plurality of third nucleic acids, wherein each of the first nucleic acids, complementary to each of the second nucleic acids, contains a sequence which is complementary to a site of each of the third nucleic acids, and the number of the first nucleic acids and the number of the second nucleic acids are each 1 to 1016 times that of the third nucleic acids; and
denaturing and localizing to a planar surface the first, second, and third nucleic acids, thereby facilitating formation of a crosslinked nucleic acid complex, which, when excited at 518 nm after staining with ethidium bromide, emits fluorescence at 605 nm with an intensity at least 10 times that of non-crosslinked first, second, and third nucleic acids.
0 Assignments
0 Petitions
Accused Products
Abstract
A process for crosslinking polynucleotide molecules that includes (1) providing a plurality of first nucleic acids, a plurality of second nucleic acids, and a plurality of third nucleic acids, in which each of the first nucleic acids, complementary to each of the second nucleic acids, contains a sequence which is complementary to a site of each of the third nucleic acids, and the number of the first nucleic acids and that of the second nucleic acids are each 1 to 1016 times that of the third nucleic acids; and (2) denaturing and localizing the pluralities of nucleic acids to a planar surface to facilitate formation of a crosslinked nucleic acid complex. Also disclosed is a solvent system, which includes a hydrophobic organic solvent and a chaotropic aqueous solvent, for conducting the above-described process.
21 Citations
27 Claims
-
1. A nucleic acid complex prepared by a process, comprising:
-
providing a plurality of first nucleic acids, a plurality of second nucleic acids, and a plurality of third nucleic acids, wherein each of the first nucleic acids, complementary to each of the second nucleic acids, contains a sequence which is complementary to a site of each of the third nucleic acids, and the number of the first nucleic acids and the number of the second nucleic acids are each 1 to 1016 times that of the third nucleic acids; and
denaturing and localizing to a planar surface the first, second, and third nucleic acids, thereby facilitating formation of a crosslinked nucleic acid complex, which, when excited at 518 nm after staining with ethidium bromide, emits fluorescence at 605 nm with an intensity at least 10 times that of non-crosslinked first, second, and third nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
- 17. A multiphasic system comprising a hydrophobic organic solvent and a chaotropic aqueous solvent separate in two phases, and a plurality of nucleic acids at the interface between the organic and aqueous solvents, wherein the organic and aqueous solvents facilitate denaturation and localization of the nucleic acids.
Specification
-
- Resources
-
Current AssigneeChang-Ning J. Wang
-
Original AssigneeChang-Ning J. Wang
-
InventorsWang, Chang-Ning J.
-
Application NumberUS11/069,631Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/6CPC Class CodesC12Q 1/6816 characterised by the detect...C12Q 1/6839 Triple helix formation or o...C12Q 1/706 for hepatitisC12Q 2523/113 Denaturating agentsC12Q 2537/119 Triple helix formationC12Q 2545/114 involving a quantitation stepC12Q 2563/173 staining/intercalating agen...
