Methods, compositions, devices, and kits for detecting mastitis
First Claim
Patent Images
1. A device adapted for the detection of mastitis in an animal, comprising:
- (a) a vessel to hold a milk sample;
(b) a first binding agent; and
(c) a second binding agent, wherein the first binding agent binds a mastitis-associated molecule, and wherein the second binding agent comprises a detectable label.
1 Assignment
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Accused Products
Abstract
The present invention includes compositions, kits and methods useful for the detection of mastitis in an animal. These agents and methods are primarily directed to a method of detecting the presence of mastitis, including sub-clinical mastitis, in cows, involving incubating a sample of milk from the cow with an agent that binds to lactoferrin such as, e.g., a monoclonal antibody specific for lactoferrin, and then detecting bound lactoferrin. The invention includes lateral-flow immunoassay methods and devices for assessing the presence of lactoferrin in milk samples.
39 Citations
41 Claims
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1. A device adapted for the detection of mastitis in an animal, comprising:
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(a) a vessel to hold a milk sample;
(b) a first binding agent; and
(c) a second binding agent, wherein the first binding agent binds a mastitis-associated molecule, and wherein the second binding agent comprises a detectable label. - View Dependent Claims (3, 4, 9, 13, 14)
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2. (canceled)
- 5. (canceled)
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7. (canceled)
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10. (canceled)
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15. (canceled)
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16. A kit adapted for the detection of mastitis in an animal, comprising:
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(a) a first binding agent;
(b) a second binding agent; and
(c) instructions for the use of the kit, wherein said first binding agent specifically bind a mastitis-associated molecule. - View Dependent Claims (17)
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- 18. (canceled)
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20. (canceled)
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23-24. -24. (canceled)
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26-27. -27. (canceled)
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28. A method adapted for detecting mastitis in an animal, comprising:
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(a) incubating a sample of milk from an animal with a labeled binding agent that binds a mastitis-associated molecule; and
(b) detecting binding agent bound to the sample, thereby detecting mastitis in the animal. - View Dependent Claims (31)
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29-30. -30. (canceled)
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32-36. -36. (canceled)
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37. An analytical test device for detecting the presence of mastitis in an animal, comprising:
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(a) a hollow casing having a liquid sample application aperture and a means permitting observation of a test result; and
(b) a test strip comprising a dry porous material contained within said hollow casing, said test strip communicating directly or indirectly with the exterior of said hollow casing through said liquid sample application aperture to receive applied liquid sample, said test strip having a capture line observable via said means permitting observation, said test strip, in the dry unused state, containing a labeled agent capable of specifically binding lactoferrin to form a first complex of said labeled agent and said bacteria, wherein said labeled agent is dry on said test strip prior to use and is released and mobile upon application of said applied liquid sample, and said test strip containing in said capture line a means for specifically binding said first complex, said means for binding being immobilized in said capture line;
wherein said binding means binds said first complex to form a second complex, said second complex being observable via said means permitting observation, thereby to indicate the presence of mastitis in the animal.
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38. (canceled)
- 39. The device of claim 3637, further comprising a control line downstream from said capture line in said dry porous carrier to indicate that said liquid sample is conveyed beyond said capture line, and a control line observation aperture in said casing, said control line also being observable from outside said hollow casing through said control line observation aperture.
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41-44. -44. (canceled)
Specification