Method and system for digital image based flourescent in situ hybridization (FISH) analysis
First Claim
1. An automated method for fluorescence in situ hybridization (FISH) analysis, comprising:
- selecting a plurality of regions of interest in a digital image of a biological tissue sample to which a fluorescent compound has been applied;
detecting a plurality of luminance signals from a plurality of objects of interest in the selected plurality of regions of interest;
grouping the detected plurality of luminance signals from the plurality of objects of interest into a plurality of sets of signals;
forming a plurality of clusters of signals from the plurality of sets of signals; and
analyzing the plurality of clusters of signals to determine a medical conclusion.
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Abstract
A method and system for automated digital fluorescent in situ hybridization (FISH) image analysis. Luminance parameters from a digital image of a biological tissue sample to which a fluorescent compound (e.g., LSI-HER-2/neu and CEP-17 dyes) have been applied are analyzed to determine plural regions of interest. Fluorescent color signals in the plural regions of interest including plural cell nuclei are identified, classified and grouped into plural groups. Each of the plural groups is validated based on pre-defined conditions. A medical diagnosis or prognosis or medical, life science or biotechnology experiment conclusion determined using a count of plural ratios of validated fluorescent color signals within each of the cell nuclei within the plural groups.
61 Citations
29 Claims
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1. An automated method for fluorescence in situ hybridization (FISH) analysis, comprising:
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selecting a plurality of regions of interest in a digital image of a biological tissue sample to which a fluorescent compound has been applied;
detecting a plurality of luminance signals from a plurality of objects of interest in the selected plurality of regions of interest;
grouping the detected plurality of luminance signals from the plurality of objects of interest into a plurality of sets of signals;
forming a plurality of clusters of signals from the plurality of sets of signals; and
analyzing the plurality of clusters of signals to determine a medical conclusion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An automated method for fluorescence in situ hybridization (FISH) analysis, comprising:
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grouping a plurality of colored fluorescent signals in a digital image of a biological tissue sample to which a fluorescent compound has been applied into a plurality of component groups if a distance between a pair of the plurality of colored fluorescent signals is less than a pre-determined threshold;
splitting the plurality of component groups into a plurality of clusters for each individual cell nucleus identified in the digital image; and
validating the plurality of clusters of signals each individual cell nucleus; and
counting a plurality of ratios of colored fluorescent colors signals within the plurality of clusters to determine a medical prognosis or diagnosis. - View Dependent Claims (19, 20, 21)
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22. An automated method for fluorescence in situ hybridization (FISH) analysis, comprising:
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analyzing luminance values of a plurality of pixels from a digital image of a biological sample to which a fluorescent compound has been applied to segment the digital image into a plurality of cell nuclei and a background portion;
grouping a plurality of fluorescent color signals from the segmented plurality of cell nuclei into a plurality groups of signals; and
determining a medical conclusion based on different color signals present in each of the plurality of groups of signals. - View Dependent Claims (23, 24, 25, 26, 27)
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28. An automated system for fluorescence in situ hybridization (FISH) analysis, comprising, in combination:
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a means for selecting a plurality of regions of interest in a digital image of a biological tissue sample to which a fluorescent compound has been applied;
a means for detecting a plurality of luminance signals from a plurality of objects of interest in the selected plurality of regions of interest;
a means for grouping the detected plurality of luminance signals from the plurality of objects of interest into a plurality of sets of signals; and
a means for forming a plurality of clusters of signals from the plurality of sets of signals;
a means for analyzing the plurality of clusters of signals to determine a medical conclusion; and
a means for creating one or more reports related to the medical conclusion, for presenting the digital image and the one or more types of reports generated for the medical conclusion on a graphical user interface.
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29. An automated system for fluorescence in situ hybridization (FISH) analysis, comprising, in combination:
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a database including a plurality of digital images of a plurality of biological samples to which a fluorescent compound has been applied;
a software module for analyzing luminance values of a plurality of pixels from a digital image of a biological sample to which a fluorescent compound has been applied to segment a digital image into a plurality of cell nuclei and a background portion, for identifying and grouping fluorescent color signals from the segmented plurality of cell nuclei into a plurality groups of signals, for validating the plurality of groups of signals for each cell nucleus in the plurality of cell nuclei and for determining a medical conclusion based on different color signals present in each of the plurality of groups of signals; and
a software module for generating one or more reports related to the medical conclusion, for presenting a digital image and the one or more types of reports generated for the conclusion on a graphical user interface.
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Specification