Pipetting system with selective pipette tip loading

• US 20050265900A1
• Filed: 05/27/2004
• Published: 12/01/2005
• Est. Priority Date: 05/27/2004
• Status: Abandoned Application

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Owens, Chad A., Gard, Douglas J.
• Application Number:
  US10/856,638
• Publication Number:
  US 20050265900A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/023 adapted for different sizes of tubes, tips or container
• CPC Class Codes: B01L 3/0234 Repeating pipettes, i.e. for dispensing multiple doses from a single charge
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 9/543 for disposable pipette tips, e.g. racks or cassettes
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom

Abstract

A system for selectively loading pipettes onto a multichannel pipettor using a pipette holder which projects the engagement ends of selected pipettes further away from a support surface compared with a remainder of pipettes retained in the holder.

Claims

1. A pipetting system comprising:

(a) a plurality of pipettes of approximately the same size, wherein each of the pipettes comprises an engagement end and a dispensing end;

(b) a movable pipette head for holding the pipettes;

(c) a framework having a first receiving surface for holding the pipettes, wherein the framework is adapted so that the engagement ends of a selected portion of the pipettes placed in the framework project further from the first receiving surface than the engagement ends of a remaining portion of the pipettes placed in the framework; and

(d) a controller for controlling movement of the pipette head so that the pipette head picks up only the selected portion of the pipettes from the framework.

2. The system of claim 1, wherein the framework comprises a second receiving surface for holding the selected portion of pipettes, the second receiving surface being elevated with respect to the first receiving surface.

3. The system of claim 2, wherein the second receiving surface is removably attached to the first receiving surface.

4. The system of claim 1, wherein the first receiving surface comprises first openings for holding the selected portion of pipettes and second openings for holding the remaining portion of pipettes, and wherein the circumference of the first openings is smaller than the circumference of the second openings.

5. The system of claim 1, wherein the first receiving surface comprises first openings for holding the selected portion of pipettes and second openings for holding the remaining portion of pipettes, and wherein the framework comprises inserts in the first openings.

6. The system of claim 5, wherein the inserts reduce the circumference of the first openings.

7. The system of claim 5, wherein the inserts provide a support surface which is elevated with respect to the second openings.

9. The system of claim 1, wherein the pipettes are pipette tips.

8. (canceled)

10. A pipetting system comprising:

(a) a plurality of pipettes of approximately the same size, wherein each pipette comprises an engagement end and a dispensing end;

(b) a movable pipette head for holding the pipettes;

(c) a source of pipettes maintaining the pipettes in a configuration for being picked up by the pipette head;

(d) a framework having a first receiving surface for holding pipettes, wherein the engagement ends of a selected portion of the pipettes placed in the framework project further from the first receiving surface than the engagement ends of a remaining portion of the pipettes placed in the framework; and

(e) a controller for controlling movement of the pipette head for transporting pipettes from the source to the framework and for picking up from the framework only the selected portion of pipettes.

11. A method for pipetting comprising the steps of:

(a) placing a plurality of pipettes of approximately the same size in a support having a first receiving surface for holding pipettes, wherein each pipette comprises an engagement end and a dispensing end, and wherein a selected portion of the pipettes placed in the support have engagement ends that project further from the first receiving surface than a second portion of the pipettes;

(b) removing only the selected portion of the pipettes from the support; and

(c) after step (b), performing pipetting operations with at least some of the removed pipettes.

12. The method of claim 11, wherein step (b) comprises loading the pipettes from the support onto a pipette head.

13. The method of claim 11, wherein step (c) comprises withdrawing fluid from a microplate.

14. The method of claim 11, wherein step (c) comprises dispensing fluid to a microplate.

15. A method for pipetting comprising the steps of:

(a) picking up a plurality of pipettes of approximately the same size with a pipette head from a source of pipettes that maintains the pipettes in a configuration for pickup by the pipette head, wherein each pipette comprises an engagement end and a liquid dispensing end;

(b) placing the picked up pipettes in a framework having a first receiving surface for holding pipettes, wherein a selected portion of the pipettes placed in the framework have engagement ends that project further from the receiving surface than the engagement ends of a second portion of pipettes;

(c) picking up only the selected portion of the pipettes from the framework with the pipette head;

(d) after step (c), performing pipetting operations with at least some of the pipettes in the pipette head;

(e) after step (d), removing the pipettes from the pipette head;

(f) after step (e), picking up the second portion of the pipettes from the framework with a pipette head; and

(g) after step (f), placing the picked up second portion of the pipettes in the framework so that a selected portion of such pipettes placed in the framework have engagement ends that project further from the receiving surface than the engagement ends of the remainder of the pipettes.

16. The method of claim 15, wherein the framework comprises a second receiving surface for holding the selected portion of pipettes, the second receiving surface being elevated with respect to the first receiving surface, and wherein the second receiving surface is removably attached to the first receiving surface, comprising the step of removing the second receiving surface from the first receiving surface after step (c).

17. A pipetting system comprising:

(a) a plurality of pipettes of approximately the same size, each pipette comprising an engagement end, a dispensing end, and a body having first and second sections, the first section being closer to the dispensing end than the second section; and

(b) a holder for holding the pipettes, the holder having a support surface with a plurality of first openings and second openings,

wherein the first openings are configured for engaging the first section of the pipettes with the engagement ends of the pipettes projecting away from the support, and

wherein the second openings are configured for engaging the second sections but not the first sections of the pipettes with the engagement ends of the pipettes projecting away from the support surface, whereby the engagement ends of the pipettes engaged by the first openings project further away from the support surface than do the pipettes engaged by the second openings.

18. The pipetting system of claim 17, wherein each pipette body is tapered with the circumference of the first section being smaller than the circumference of the second section, and the circumference of the first openings is smaller than the circumference of the second openings.

19. The pipetting system of claim 17, wherein each pipette body is tapered with the circumference of the first section being smaller than the circumference of the second section, the circumference of the first openings being substantially the same as the circumference of the second openings, and wherein the system comprises an insert in each of the first openings to reduce their circumference.

20. The pipetting system of claim 17, wherein each pipette body is tapered with the circumference of the first section being smaller than the circumference of the second section, the circumference of the first openings being substantially the same as the circumference of the second openings, and wherein the system comprises an insert in each of the first openings, wherein the insert is adapted to engage a pipette so that it projects further away from the support surface than pipettes engaged by the first openings.