Nanogene therapy for cell proliferation disorders
First Claim
1. A method for treating a cell proliferation disorder in a subject, comprising administering a therapeutically effective amount of particles to the subject, wherein the particles comprise:
- a polynucleotide encoding an interferon, an interferon-inducible molecule, or both; and
a chitin-containing component associated with the polynucleotide, wherein the polynucleotide is expressed in the subject and cell proliferation is reduced.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention concerns particles comprising a chitin component, such as chitosan or a derivative thereof, associated with a polynucleotide encoding an interferon (IFN) molecule, 2-5′ oligoadenylate synthetase (2-5 AS), or a combination thereof. Preferably, the chitin component comprises chitosan or a derivative thereof. The particles of the invention are useful for delivery and expression of the interferon-encoding and/or 2-5 AS-encoding polynucleotide within a host in vitro or in vivo. The invention further concerns pharmaceutical compositions comprising particles of the invention and a pharmaceutically acceptable carrier, and a method for producing particles of the present invention. The present invention further pertains to a method of inducing apoptosis in a cancer cell, such as a lung cancer cell, by contacting a target cancer cell in vitro or in vivo with an effective amount of particles of the invention. In one embodiment, a therapeutically effective amount of particles are administered to target cancer cells within a patient in vivo, for treatment of cancer, such as lung cancer. The particles and therapeutic methods of the invention provide anti-metastatic and anti-cancer therapeutics for cancer patients, particularly lung cancer patients.
-
Citations
31 Claims
-
1. A method for treating a cell proliferation disorder in a subject, comprising administering a therapeutically effective amount of particles to the subject, wherein the particles comprise:
- a polynucleotide encoding an interferon, an interferon-inducible molecule, or both; and
a chitin-containing component associated with the polynucleotide, wherein the polynucleotide is expressed in the subject and cell proliferation is reduced. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- a polynucleotide encoding an interferon, an interferon-inducible molecule, or both; and
-
19. A method of inducing apoptosis in a cancer cell, comprising contacting a target cancer cell in vitro or in vivo with an effective amount of particles comprising:
- a polynucleotide encoding an interferon, an interferon-inducible molecule, or both; and
a chitin-containing component associated with the polynucleotide, wherein the polynucleotide is expressed in the cancer cell and apoptosis is induced. - View Dependent Claims (20, 21, 22)
- a polynucleotide encoding an interferon, an interferon-inducible molecule, or both; and
-
23. A particle comprising a polynucleotide encoding an interferon, an interferon-inducible molecule, or both;
- and a chitin-containing component associated with the polynucleotide.
- View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31)
Specification