Combinatorial decoding of random nucleic acid arrays
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Abstract
The invention provides methods and compositions for combinatorially decoding arrays.
92 Citations
26 Claims
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1. -23. (canceled)
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24. A method of decoding an array composition comprising
a) providing an array composition comprising: -
i) a substrate with a surface comprising discrete sites; and
ii) a population of microspheres comprising at least a first and a second subpopulation distributed on said discrete sites, wherein each subpopulation comprises an identifier nucleic acid sequence comprising;
1) a primer sequence; and
2) a decoding sequence adjacent to said primer sequence;
wherein said microspheres are distributed on said surface; and
b) adding to said array composition a first probe of a set of combinatorial decoding probes each probe comprising;
i) a priming sequence complementary to said primer sequence;
ii) a least one decoding nucleotide that will basepair with at least one nucleotide of at least one of said decoding sequences, said decoding nucleotide comprising a label; and
c) detecting the presence of said label.
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25. The method according to claim 25, further comprising:
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d) adding to said array composition a second probe of a set of combinatorial decoding probes comprising;
i) a priming sequence complementary to said primer sequence;
ii) at least one decoding nucleotide that will basepair with at least one nucleotide of at least one of said decoding sequences, said decoding nucleotide comprising a label; and
e) detecting the presence of said label.
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26. (canceled)
Specification