Magnetic device for isolation of cells and biomolecules in a microfluidic environment
First Claim
1. A device for retaining one or more desired analytes in a sample, said device comprising a first region of magnetic obstacles disposed in a channel and a plurality of magnetic particles attached to at least one of said obstacles by a magnetic interaction.
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Accused Products
Abstract
The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.
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Citations
54 Claims
- 1. A device for retaining one or more desired analytes in a sample, said device comprising a first region of magnetic obstacles disposed in a channel and a plurality of magnetic particles attached to at least one of said obstacles by a magnetic interaction.
- 18. A device for retaining one or more desired analytes in a sample, said device comprising a channel comprising a plurality of magnetic obstacles, wherein said obstacles comprise a plurality of magnetic particles, and said magnetic particles comprise a capture moiety capable of binding said one or more analytes.
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19. A device for retaining one or more desired analytes in a sample, said device comprising a channel comprising a plurality of magnetic obstacles, wherein said obstacles comprise a plurality of magnetic particles, and wherein said magnetic obstacles are disposed such that at least a portion of said one or more analytes cannot pass between said obstacles.
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22. A method for retaining a first type of analyte in a sample, said method comprising the steps of:
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(a) providing a sample comprising at least a first and a second type of analyte and a device comprising;
(i) a first region of magnetic obstacles disposed in a channel; and
(ii) a plurality of magnetic particles attached to at least one of said obstacles by a magnetic interaction; and
(b) introducing said sample into said device, wherein said first type of analyte is retained in said device by interaction with at least one of said obstacles. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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23. A method for retaining a first type of analyte in a sample, said method comprising the steps of:
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(a) providing a sample comprising at least a first and a second type of analyte and a device comprising a first region of magnetic obstacles disposed in a channel, wherein said obstacles comprise a plurality of magnetic particles; and
(b) introducing said sample into said device, wherein said first type of analyte is retained in said device by interaction with at least one of said obstacles.
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Specification