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Method for identifying activators of gene transcription

  • US 20050266447A1
  • Filed: 04/07/2005
  • Published: 12/01/2005
  • Est. Priority Date: 04/19/2004
  • Status: Abandoned Application
First Claim
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1. A method for identifying a nucleotide sequence that encodes a polypeptide that modulates the activity of a transcriptional regulatory region, said method comprising:

  • (a) providing a population of polynucleotide constructs, wherein each of said polynucleotide constructs in said population comprises the transcriptional regulatory region operably linked to a candidate nucleotide sequence;

    (b) introducing said population of polynucleotide constructs into a population of host cells; and

    , (c) screening for a modulation in the expression level of each candidate nucleotide sequence, and thereby identifying a nucleotide sequence that encodes a polypeptide that modulates the activity of said transcriptional regulatory region.

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