Progenitor cells, methods and uses related thereto
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Abstract
The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.
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Citations
78 Claims
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1-30. -30. (canceled)
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31. A method of isolating progenitor cells comprising:
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i) obtaining pancreatic ductal cells;
ii) culturing said pancreatic cells in a suitable nutrient medium;
iii) isolating a population of progenitor cells from said culture. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40)
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- 41. (canceled)
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42. A method for stimulating the ex vivo proliferation of mammalian pancreatic β
- -islet cells, comprising the steps of;
(a) preparing a primary culture of mammalian pancreatic cells; and
,(b) contacting said primary culture cells with an effective concentration of a cAMP agonist, wherein the effective concentration is an amount sufficient to induce the primary culture to differenitate to β
-islet cells. - View Dependent Claims (43)
- -islet cells, comprising the steps of;
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47. A method for stimulating the ex vivo proliferation of human adult pancreatic beta-cells, comprising the steps of:
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(a) preparing a monolayer culture of primary human adult pancreatic cells; and
(b) culturing said cells with an effective concentration of a growth factor and a cAMP agonist, wherein the effective concentration is an amount sufficient to induce the primary culture to produce insulin-producing cells.
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48-54. -54. (canceled)
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55. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
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obtaining a cell suspension from an animal tissue, wherein said cell suspension comprises at least one progenitor cell;
treating the cell suspension with a growth factor preparation; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 71, 72, 73, 74, 75, 76, 77, 78)
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69. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
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providing an animal tissue;
disrupting said animal tissue so as to obtain a cell suspension comprising at least one progenitor cell; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population. - View Dependent Claims (70)
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Specification