Percutaneous chemical sensor based on fluorescence resonant energy transfer (FRET)
First Claim
1. A device for detecting an analyte from within a patient'"'"'s body, comprising:
- a) an optical fiber having;
a first end; and
a second end configured to intermittently connect and disconnect to an analyzer;
and b) a biosensing material attached to the first end comprising;
a polymer matrix;
at least one receptor molecule attached to the polymer matrix and labeled with a first detector molecule; and
at least one competitive binding molecule attached to the polymer matrix and labeled with a second detector molecule, wherein the at least one receptor molecule is capable of interacting with the at least one attached competitive binding molecule and with at least one analyte from the patient'"'"'s body.
1 Assignment
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Accused Products
Abstract
A biosensing device for detecting biological analytes, and methods of use and manufacture, are disclosed. The device includes a biosensing element that can remain implanted for extended periods of time. The biosensing element is connected to an optical fiber terminating outside of the body. The optical fiber is also connected to an information analyzer. The information analyzer directs light through the optical fiber into the biosensing element. The light excites fluorophores, created by a chemical reaction between analytes and biosensing material within the biosensing element. Emitted fluorescent light is redirected through the optical fiber to the information analyzer. Detectors detect the deflected fluorescent emissions and, according to their determined wavelength, report the presence or quantity of specific analytes to the patient on an external display.
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Citations
56 Claims
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1. A device for detecting an analyte from within a patient'"'"'s body, comprising:
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a) an optical fiber having;
a first end; and
a second end configured to intermittently connect and disconnect to an analyzer;
and b) a biosensing material attached to the first end comprising;
a polymer matrix;
at least one receptor molecule attached to the polymer matrix and labeled with a first detector molecule; and
at least one competitive binding molecule attached to the polymer matrix and labeled with a second detector molecule, wherein the at least one receptor molecule is capable of interacting with the at least one attached competitive binding molecule and with at least one analyte from the patient'"'"'s body. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system for detecting an analyte from within a patient'"'"'s body, comprising:
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a) an optical fiber comprising;
a first end; and
a second end configured to intermittently connect and disconnect to an analyzer;
b) a biosensing material attached to the first end comprising;
a polymer matrix;
at least one receptor molecule attached to the polymer matrix and labeled with a first detector molecule; and
at least one competitive binding molecule attached to the polymer matrix and labeled with a second detector molecule, wherein the at least one receptor molecule is capable of interacting with the at least one attached competitive binding molecule and with at least one analyte from the patient'"'"'s body;
and c) an analyzer that is configured to rapidly and intermittently connect and disconnect to the second end of the optical fiber, and that is configured to emit light into the optical fiber, receive light from the biosensing material, and process information from the received light. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method of manufacturing an implantable biosensing device, comprising:
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a) modifying the surface of a first end of an optical fiber to create an adhesion region;
b) submerging the first end of the optical fiber into a matrix precursor solution;
c) delivering ultraviolet light through a second end of the optical fiber; and
d) removing the first end of the optical fiber from the matrix precursor solution. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42)
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43. A method of detecting an analyte from within a patient'"'"'s body, comprising:
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a) implanting an optical fiber having an implanted and free end within the patient'"'"'s body such that the implanted end lies within the percutaneous region and the free end protrudes from the patient'"'"'s body;
b) allowing the implanted end of the optical fiber to remain in the percutaneous region of the body without removal for at least seven days;
c) allowing the free end of the optical fiber to remain unconnected to any device for a substantial portion of the at least seven days while the implanted end remains in the percutaenous region of the body;
d) connecting and disconnecting the free end to a measuring instrument; and
e) testing the analyte with the measuring instrument while it is connected to the free end;
wherein the optical fiber comprises;
a) a polymer matrix;
b) at least one receptor molecule attached to the polymer matrix and labeled with a first detector molecule; and
c) at least one competitive binding molecule attached to the polymer matrix and labeled with a second detector molecule, wherein the at least one receptor molecule is capable of interacting with the at least one attached competitive binding molecules and with at least one analyte from the patient'"'"'s body. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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Specification