Methods and devices for targeting a site in a mammal and for removing species from a mammal
First Claim
1. A method of removing at least one species from a mammal, the method comprising a first step of confining in a device a binding compound, the binding compound having affinity for a binding partner, a second step of preparing a plurality of affinity binders, each of said affinity binders comprising a first portion comprising the binding partner and a second portion adapted to bind selectively with a species, and thereafter a step of selecting at least one of the affinity binders and introducing said at least one affinity binder into the device so as to cause the binding partner to bind to the binding compound, and a step of connecting the device to a fluid source in a mammal.
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Accused Products
Abstract
Methods and devices for improved targeting to a site in an organism, particularly to a tumor target site and for extracorporeal affinity adsorption, particularly in the treatment of cancer, atherosclerosis, including coronary artery disease, unstable angina, other acute ischemic syndromes and idiopathic dilated cardiac myopathy. In one aspect of the invention, a combination is provided comprising an extracorporeal device (1) having contained therein a binding compound (11) bound to a carrier (9), the binding compound having affinity for a binding partner, and a plurality of affinity binders (15), each of said affinity binders comprising a first portion (19) comprising the binding partner and a second portion (17) adapted to bind selectively with a species, the second portions of each of said affinity binders differing from each other.
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Citations
82 Claims
- 1. A method of removing at least one species from a mammal, the method comprising a first step of confining in a device a binding compound, the binding compound having affinity for a binding partner, a second step of preparing a plurality of affinity binders, each of said affinity binders comprising a first portion comprising the binding partner and a second portion adapted to bind selectively with a species, and thereafter a step of selecting at least one of the affinity binders and introducing said at least one affinity binder into the device so as to cause the binding partner to bind to the binding compound, and a step of connecting the device to a fluid source in a mammal.
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2. (canceled)
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22. A method of making a binding device comprising a first step of confining in the device a binding compound, the binding compound having affinity for a binding partner, a second step of preparing a plurality of affinity binders, each of said affinity binders comprising a first portion comprising the binding partner and a second portion adapted to bind selectively with a species, and thereafter a step of selecting at least one of the affinity binders and introducing said at least one affinity binder into the device so as to cause the binding partner to bind to the binding compound, wherein at least one of the affinity binders comprises a first portion having affinity to the binding compound and a second portion having affinity to at least one of the group consisting of TNF alpha, IL-6, CD3+ DR+ T cells, CD4+ CD28null T cells, CD3+, CD56+, DM1, VGO1, LAK1, CRP, INF gamma, CA, NAB, CA-NAB, TGF β
- , P15E, Sialomucin, TH2 T cell epitope, tumor infiltrating lymphocyte (TIL) marker, lymphokine activated killer cell (LAK) marker, Interleukin 10 (IL-10), prostaglandin E2 (PGE2), mucin, suppressive E receptor (SER), immunosuppressive acidic protein (IAP), adhesion molecules, sR TNF alpha, sR TNF beta, sR IL-1, sR IL-2, sR IL-6, sR INF gamma, heat shock protein (HSP), antibodies to oxidized LDL (Ab-OxLDL), antibodies to HSP, CRP, triglycerides, IL-2, metalloproteinases, other proteinases, fibrinogen, creatine kinase, IL-1-Beta, IL-1-Ra, PDGF, angiotensin II, MCSF, pregnancy associated plasma protein A (PAPPA), antibodies specific to any of the following;
the β
1 adrenergic receptor, ADP-ATP carrier, alpha cardiac myosin heavy chain isoform, beta cardiac myosin heavy chain isoform, G Protein coupled receptors, and heart mitochondria, oxidants, and toxins selected from the group consisting of botulinum toxin, tetanus toxin, ricin toxin, ricin A peptide toxin, endotoxin, sulfur mustard, prescription drugs, over-the-counter drugs, drugs of abuse, chemical poisons, and toxic metabolites thereof.
- , P15E, Sialomucin, TH2 T cell epitope, tumor infiltrating lymphocyte (TIL) marker, lymphokine activated killer cell (LAK) marker, Interleukin 10 (IL-10), prostaglandin E2 (PGE2), mucin, suppressive E receptor (SER), immunosuppressive acidic protein (IAP), adhesion molecules, sR TNF alpha, sR TNF beta, sR IL-1, sR IL-2, sR IL-6, sR INF gamma, heat shock protein (HSP), antibodies to oxidized LDL (Ab-OxLDL), antibodies to HSP, CRP, triglycerides, IL-2, metalloproteinases, other proteinases, fibrinogen, creatine kinase, IL-1-Beta, IL-1-Ra, PDGF, angiotensin II, MCSF, pregnancy associated plasma protein A (PAPPA), antibodies specific to any of the following;
- 33. In combination, a device having contained therein a binding compound bound to a carrier, the binding compound having affinity for a binding partner, and a plurality of affinity binders, each of said affinity binders comprising a first portion comprising the binding partner and a second portion adapted to bind selectively with a species, the second portions of each of said affinity binders differing from each other.
- 41. A pharmaceutical preparation for the treatment of atherosclerosis comprising an immunogenic preparation of a molecular inflammatory factor (MIF) and a pharmaceutically acceptable pharmaceutical carrier.
- 43. An extracorporeal device, comprising at least one affinity adsorbent, said affinity adsorbent binding at least one exotoxin.
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52. A method of removing a species from a mammal comprising introducing into the mammal an affinity binder which selectively binds the species, the affinity binder including a binding partner portion having affinity for a binding compound, and thereafter removing the affinity binder by capturing the affinity binder in a device having contained therein the binding compound, wherein the species is selected from the group consisting of TNF alpha, IL-6, CD3+ DR+ T cells, CD4+ CD28null T cells, CD3+, CD56+, DM1, VGO1, LAK1, CRP, INF gamma, CA, NAB, CA-NAB, TGF β
- , P15E, Sialomucin, TH2 T cell epitope, tumor infiltrating lymphocyte (TIL) marker, lymphokine activated killer cell (LAK) marker, Interleukin 10 (IL-10), prostaglandin E2 (PGE2), mucin, suppressive E receptor (SER), immunosuppressive acidic protein (IAP), adhesion molecules, sR TNF alpha, sR TNF beta, sR IL-1, sR IL-2, sR IL-6, sR INF gamma, heat shock protein (HSP), antibodies to oxidized LDL (Ab-OxLDL), antibodies to HSP, CRP, triglycerides, IL-2, metalloproteinases, other proteinases, fibrinogen, creatine kinase, IL-1-Beta, IL-1-Ra, PDGF, angiotensin II, MCSF, pregnancy associated plasma protein A (PAPPA), antibodies specific to any of the following;
the β
1 adrenergic receptor, ADP-ATP carrier, alpha cardiac myosin heavy chain isoform, beta cardiac myosin heavy chain isoform, G Protein coupled receptors, and heart mitochondria, a targeted species bound to a targeting species, and toxins selected from the group consisting of botulinum toxin, tetanus toxin, ricin toxin, ricin A peptide toxin, sulfur mustard, prescription drugs, over-the-counter drugs, drugs of abuse, chemical poisons, and toxic metabolites thereof.
- , P15E, Sialomucin, TH2 T cell epitope, tumor infiltrating lymphocyte (TIL) marker, lymphokine activated killer cell (LAK) marker, Interleukin 10 (IL-10), prostaglandin E2 (PGE2), mucin, suppressive E receptor (SER), immunosuppressive acidic protein (IAP), adhesion molecules, sR TNF alpha, sR TNF beta, sR IL-1, sR IL-2, sR IL-6, sR INF gamma, heat shock protein (HSP), antibodies to oxidized LDL (Ab-OxLDL), antibodies to HSP, CRP, triglycerides, IL-2, metalloproteinases, other proteinases, fibrinogen, creatine kinase, IL-1-Beta, IL-1-Ra, PDGF, angiotensin II, MCSF, pregnancy associated plasma protein A (PAPPA), antibodies specific to any of the following;
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53. A species-removing device having contained therein a binding compound attached to a matrix and an affinity binder bound by affinity binding to the binding compound, the affinity binder having affinity for a species selected from the group consisting of TNF alpha, IL-6, CD3+ DR+ T cells, CD4+ CD28null T cells, CD3+, CD56+, DM1, VGO1, LAK1, CRP, INF gamma, CA, NAB, CA-NAB, TGF 13, P15E, Sialomucin, TH2 T cell epitope, tumor infiltrating lymphocyte (TIL) marker, lymphokine activated killer cell (LAK) marker, Interleukin 10 (IL-10), prostaglandin E2 (PGE2), mucin, suppressive E receptor (SER), immunosuppressive acidic protein (IAP), adhesion molecules, sR TNF alpha, sR TNF beta, sR IL-1, sR IL-2, sR IL-6, sR INF gamma, heat shock protein (HSP), antibodies to oxidized LDL (Ab-OxLDL), antibodies to HSP, CRP, triglycerides, IL-2, metalloproteinases, other proteinases, fibrinogen, creatine kinase, IL-1-Beta, IL-1-Ra, PDGF, angiotensin II, MCSF, pregnancy associated plasma protein A (PAPPA), antibodies specific to any of the following:
- the β
1 adrenergic receptor, ADP-ATP carrier, alpha cardiac myosin heavy chain isoform, beta cardiac myosin heavy chain isoform, G Protein coupled receptors, heart mitochondria, a targeted species bound to a targeting species, and toxins selected from the group consisting of botulinum toxin, tetanus toxin, ricin toxin, a ricin A peptide toxin, endotoxin, sulfur mustard, prescription drugs, over-the-counter drugs, drugs of abuse, chemical poisons, and toxic metabolites thereof. - View Dependent Claims (54, 55, 56)
- the β
- 61. A pharmaceutical preparation for reducing of at least one molecular inflammatory factor (MIF) or cellular inflammatory factor (CIF), the pharmaceutical preparation comprising a species selected from a non-catalytic polyclonal antibody, a catalytic polyclonal antibody, a non-catalytic monoclonal antibody, a catalytic monoclonal antibody, antibody fragment, a synthetic antibody fragment, an antibody analog, a chimeric monoclonal antibody, a humanized monoclonal antibody, a fragment of any of the above antibodies, including synthetic fragments and analogs of such fragments, wherein said antibody, antibody fragment or analog selectively binds said MIF or CIF and including a pharmaceutically acceptable carrier.
- 76. An extracorporeal device, comprising at least one affinity adsorbent, said affinity adsorbent binding at least one endotoxin wherein the at least one affinity adsorbent is selected from antibodies, antibody fragments, synthetic antibody binding site analogs, genetically engineered synthetic antibody binding site analogs, endotoxin receptor binding sites, synthetic or genetically engineered endotoxin receptor binding site analogs.
Specification