Arrays of magnetic particles
7 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
-
Citations
54 Claims
-
1. (canceled)
-
2. (canceled)
-
3. (canceled)
-
4. (canceled)
-
5. (canceled)
-
6. (canceled)
-
7. (canceled)
-
8. (canceled)
-
9. (canceled)
-
10. (canceled)
-
11. (canceled)
-
12. (canceled)
-
13. (canceled)
-
14. (canceled)
-
15. (canceled)
-
16. (canceled)
-
17. (canceled)
-
18. (canceled)
-
19. (canceled)
-
20. (canceled)
-
21. (canceled)
-
22. (canceled)
-
23. (canceled)
-
24. (canceled)
-
25. (canceled)
-
26. (canceled)
-
27. (canceled)
-
28. (canceled)
-
29. (canceled)
-
30. (canceled)
-
31. (canceled)
-
32. (canceled)
-
33. (canceled)
-
34. (canceled)
-
35. (canceled)
-
36. (canceled)
-
37. (canceled)
-
38. (canceled)
-
39. (canceled)
-
40. (canceled)
- 41. An array comprising a plurality of magnetic particles comprising at least two different particle populations distinguishable by a chemical characteristic associated therewith, wherein the magnetic particles are suspended in a fluid, are magnetically polarizable and are assembled in a planar array on one or more chips using a magnetic field.
-
47. A method of performing a bioassay comprising:
-
48. A method for performing a bioassay involving integration of sample preparation and parallel molecular interaction assay analysis, comprising:
-
providing an apparatus comprising a sample preparation compartment and an assay compartment, and fluidic connection for the sample preparation and the assay compartments;
providing, in the sample preparation compartment, a biological fluid containing a biomolecule of interest and a plurality of magnetic particles capable of binding to the biomolecule of interest under conditions wherein the magnetic particles to bind the biomolecules of interest;
removing the biological fluid along with unbound components of said fluid, while retaining the magnetic particles and the biomolecules bound to said particles; and
releasing said biomolecules from said magnetic particles and transporting said biomolecules from the sample preparation compartment to the assay compartment through the fluidic connection; and
performing a bioassay wherein the analyte in the bioassay comprises transported biomolecules of interest. - View Dependent Claims (49, 50, 51)
-
-
52. A method of integrating sample preparation and bioassay using magnetic particles comprising:
-
providing a plurality of magnetic particles comprising at-least two different particle populations, each population having a recognition molecule attached thereto, wherein the particles are distinguishable a chemical characteristic that uniquely identifies a biomolecule that selectively binds to the recognition molecule;
providing a biological fluid containing biomolecules and allowing said biomolecules to interact with the recognition molecules on the magnetic particles;
removing the fluid along with unbound components thereof;
transforming the biomolecules bound to the magnetic particles to produce transformed biomolecules, wherein the transformed biomolecules remain attached to the magnetic particles on which they are synthesized;
performing a bioassay using the transformed biomolecules. - View Dependent Claims (53, 54)
-
Specification