Alternatively spliced isoform of acetyl-CoA carboxylase 2 (ACC2)
First Claim
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1. A purified human nucleic acid comprising SEQ ID NO 3, or the complement thereof.
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Abstract
The present invention features nucleic acids and polypeptides encoding novel splice variant isoforms of acetyl-CoA carboxylase 2 (ACC2). The polynucleotide sequence of ACC2sv1 is provided by SEQ ID NO 3. The amino acid sequence of ACC2sv1 is provided by SEQ ID NO 4. The present invention also provides methods for using ACC2sv1 polynucleotides and proteins to screen for compounds that bind to ACC2sv1.
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20 Claims
- 1. A purified human nucleic acid comprising SEQ ID NO 3, or the complement thereof.
- 4. A purified polypeptide comprising SEQ ID NO 4.
- 6. An expression vector comprising a nucleotide sequence encoding SEQ ID NO 4, wherein said nucleotide sequence is transcriptionally coupled to an exogenous promoter.
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10. A method of screening for compounds able to bind selectively to ACC2sv1 comprising the steps of:
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(a) providing a ACC2sv1 polypeptide comprising SEQ ID NO 4;
(b) providing one or more ACC isoform polypeptides that are not ACC2sv1;
(c) contacting said ACC2sv1 polypeptide and said ACC isoform polypeptide that is not ACC2sv1 with a test preparation comprising one or more compounds; and
(d) determining the binding of said test preparation to said ACC2sv1 polypeptide and to said ACC isoform polypeptide that is not ACC2sv1, wherein a test preparation which binds to said ACC2sv1 polypeptide, but does not bind to said ACC isoform polypeptide that is not ACC2sv1, contains a compound that selectively binds said ACC2sv1 polypeptide. - View Dependent Claims (11, 12)
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13. A method for screening for a compound able to bind to or interact with a ACC2sv1 protein or a fragment thereof comprising the steps of:
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(a) expressing a ACC2sv1 polypeptide comprising SEQ ID NO 4 or fragment thereof from a recombinant nucleic acid;
(b) providing to said polypeptide a labeled ACC ligand that binds to said polypeptide and a test preparation comprising one or more compounds; and
(c) measuring the effect of said test preparation on binding of said labeled ACC ligand to said polypeptide, wherein a test preparation that alters the binding of said labeled ACC2 ligand to said polypeptide contains a compound that binds to or interacts with said polypeptide. - View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A method of screening for ACC2sv1 activity comprising the steps of:
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(a) contacting a cell expressing a recombinant nucleic acid encoding ACC2sv1 comprising SEQ ID NO 4 with a test preparation comprising one or more test compounds; and
(b) measuring the effect of said test preparation on enzyme activity.
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Specification