Method for the early detection of renal injury
First Claim
5. The method for monitoring the effectiveness of a treatment for renal tubular cell injury, comprising the steps of:
- a) obtaining a blood serum sample from a mammalian subject experiencing renal tubular cell injury;
b) determining from the serum sample the level of a biomarker selected from an immediate renal tubular cell injury biomarker, an early on-set renal tubular cell injury biomarker, and mixtures thereof, and c) evaluating the renal tubular cell injury status of the subject.
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Abstract
A method and kit for detecting the immediate or early onset of renal disease and injury, including renal tubular cell injury, utilizing NGAL as an immediate or early on-set biomarker in a sample of blood serum. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected in the blood serum following renal tubule cell injury. NGAL protein expression is detected predominantly in proximal tubule cells, in a punctuate cytoplasmic distribution reminiscent of a secreted protein. The appearance NGAL in the serum is related to the dose and duration of renal ischemia and nephrotoxemia, and is diagnostic of renal tubule cell injury and renal failure. NGAL detection is also a useful marker for monitoring the nephrotoxic side effects of drugs or other therapeutic agents.
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Citations
20 Claims
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5. The method for monitoring the effectiveness of a treatment for renal tubular cell injury, comprising the steps of:
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a) obtaining a blood serum sample from a mammalian subject experiencing renal tubular cell injury;
b) determining from the serum sample the level of a biomarker selected from an immediate renal tubular cell injury biomarker, an early on-set renal tubular cell injury biomarker, and mixtures thereof, and c) evaluating the renal tubular cell injury status of the subject. - View Dependent Claims (6, 7, 8, 9, 10)
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11. A kit for use in detecting the presence of an immediate or early onset biomarker for renal tubular cell injury in the serum of a subject, comprising:
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a) a means for acquiring a quantity of a blood serum sample;
b) a media having affixed thereto a capture antibody capable of complexing with a renal tubular cell injury biomarker selected from an immediate renal tubular cell injury biomarker, an early on-set renal tubular cell injury biomarker, and mixtures thereof; and
c) an assay for the detection of a complex of the renal tubular cell injury biomarker and the capture antibody. - View Dependent Claims (1, 2, 3, 4, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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20-1. The method according to claim 17 wherein the biomarker comprises NGAL.
Specification