Stem cell populations and methods of use
First Claim
1. A population of stem and progenitor cells that has been expanded in a cytokine cocktail, wherein said population comprises a CD34+ cell population and a CD34br cell population, and wherein said CD34br cell population has an antibody bound per cell value that is at least 1.5-fold greater than the antibody bound per cell value for said population of CD34+ cells.
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Accused Products
Abstract
The invention provides populations of expanded CD34-expressing cells and methods of use. Particular embodiments provide for defined culture media useful for growing these cells, and grafts comprising these cells. The invention finds use in methods for reconstituting, repairing, and regenerating tissue damage.
31 Citations
32 Claims
- 1. A population of stem and progenitor cells that has been expanded in a cytokine cocktail, wherein said population comprises a CD34+ cell population and a CD34br cell population, and wherein said CD34br cell population has an antibody bound per cell value that is at least 1.5-fold greater than the antibody bound per cell value for said population of CD34+ cells.
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11. A cell culture medium for expanding a population of stem and progenitor cells, said medium comprising at least 8 cytokines selected from the group consisting of fibronectin, SDF-1α
- , IL-6, SCF, IL-5, BDNF, PD-ECGF, IL-11, IL-3, EPO, Flt-3/Flk-2 ligand, BMP-4, thrombospondin, IGF-1, bFGF, angiotensin, PDGF-AB, TGF-β
1, GM-CSF, VEGF, EGF, M-CSF, and MCP-1, PlGF, hematopoietic adhesion peptide comprising the amino acid sequence VTCG, TRANCE, G-CSF, Tpo, LIF, and SCGF-α
, and combinations thereof. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
- , IL-6, SCF, IL-5, BDNF, PD-ECGF, IL-11, IL-3, EPO, Flt-3/Flk-2 ligand, BMP-4, thrombospondin, IGF-1, bFGF, angiotensin, PDGF-AB, TGF-β
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21. A method of producing an expanded population of stem and progenitor cells, said method comprising culturing a population of stem and progenitor cells in a cell culture medium comprising at least 8 cytokines selected from the group consisting of fibronectin, SDF-1α
- , IL-6, SCF, IL-5, BDNF, PD-ECGF, IL-11, IL-3, EPO, Flt-3/Flk-2 ligand, BMP-4, thrombospondin, IGF-1, bFGF, angiotensin, PDGF-AB, TGF-β
1, GM-CSF, VEGF, EGF, M-CSF, and MCP-1, PlGF, hematopoietic adhesion peptide comprising the amino acid sequence VTCG, TRANCE, G-CSF, Tpo, LIF, and SCGF-α
, and combinations thereof. - View Dependent Claims (22)
- , IL-6, SCF, IL-5, BDNF, PD-ECGF, IL-11, IL-3, EPO, Flt-3/Flk-2 ligand, BMP-4, thrombospondin, IGF-1, bFGF, angiotensin, PDGF-AB, TGF-β
- 23. A method of transplanting a population of stem and progenitor cells comprising administering an expanded population of stem and progenitor cells to a patient in need thereof, wherein said expanded population comprises a CD34+ cell population and a CD34br cell population, and wherein said CD34br cell population has an antibody bound per cell value that is at least 1.5-fold greater than the antibody bound per cell value for said population of CD34+ cells.
Specification