DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT AND REACTION
First Claim
Patent Images
1. A method of conducting a reaction within at least one plug comprising the steps of:
- introducing a carrier-fluid into a device comprising a first channel in fluid communication with a first;
simultaneously introducing at least two streams of plug-fluids into the first inlet so that at least one plug forms in the carrier-fluid after the streams contact the carrier-fluid;
wherein;
a first plug-fluid comprises a first reagent;
a second plug-fluid comprises a second reagent different from the first reagent, each plug-fluid is immiscible with the carrier-fluid; and
each plug comprises both the first and second plug-fluids so that the reagents may undergo the reaction, each plug is substantially surrounded by carrier.
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Abstract
The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
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Citations
83 Claims
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1. A method of conducting a reaction within at least one plug comprising the steps of:
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introducing a carrier-fluid into a device comprising a first channel in fluid communication with a first;
simultaneously introducing at least two streams of plug-fluids into the first inlet so that at least one plug forms in the carrier-fluid after the streams contact the carrier-fluid;
wherein;
a first plug-fluid comprises a first reagent;
a second plug-fluid comprises a second reagent different from the first reagent, each plug-fluid is immiscible with the carrier-fluid; and
each plug comprises both the first and second plug-fluids so that the reagents may undergo the reaction, each plug is substantially surrounded by carrier. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 25, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 55, 58, 59, 60)
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8. (canceled)
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13. (canceled)
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15-17. -17. (canceled)
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19-24. -24. (canceled)
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26-28. -28. (canceled)
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46-54. -54. (canceled)
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56-57. -57. (canceled)
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61. A method of conducting a reaction within at least one plug comprising the steps of:
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introducing a carrier-fluid into a device comprising a first channel in fluid communication with a first inlet;
simultaneously introducing at least two streams of plug-fluids into the first inlet so that at least one plug forms in the carrier fluid at a junction of the first inlet and the first channel;
wherein;
a first plug-fluid comprises a first reagent;
a second plug-fluid comprises a second reagent different from the first reagent;
each plug-fluid is immiscible with the carrier-fluid; and
each plug comprises both the first and second plug-fluids so that the reagents may undergo the reaction;
each plug is substantially surrounded by carrier.
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62. A method of conducting a reaction within at least one plug comprising the steps of:
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introducing a carrier-fluid into a device comprising a first channel in fluid communication with a first inlet and a second inlet;
introducing a stream of a first plug-fluid into the first inlet and simultaneously introducing a stream of a second plug-fluid into the second inlet so that at least one plug forms in the carrier-fluid after the first and second plug-fluids contact the carrier fluid;
wherein;
a first plug-fluid comprises a first reagent;
a second plug-fluid comprises a second reagent different from the first reagent;
each plug-fluid is immiscible with the carrier-fluid;
each plug comprises both the first and second plug-fluids so that the reagents can react; and
each plug is substantially surrounded by carrier. - View Dependent Claims (63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81)
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82. A method of conducting a reaction within at least one plug comprising the steps of:
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introducing a carrier-fluid into a device comprising a first channel in fluid communication with a first inlet and a second inlet;
introducing a stream of a first plug-fluid into the first inlet and simultaneously introducing a stream of a second plug-fluid into the second inlet so that at least one plug forms in the carrier fluid at a junction area of the first and second inlets and the first channel;
wherein;
a first plug-fluid comprises a first reagent;
a second plug-fluid comprises a second reagent different from the first reagent;
each plug-fluid is immiscible with the carrier-fluid;
each plug comprises both the first and second plug-fluids so that the reagents can react; and
each plug is substantially surrounded by carrier. - View Dependent Claims (83)
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Specification