Pufa polyketide synthase systems and uses thereof
First Claim
1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- a) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, and biologically active fragments thereof;
b) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ BD NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ BD NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, and biologically active fragments thereof;
c) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to at least 500 consecutive amino acids of said amino acid sequence of (a), wherein said amino acid sequence has a biological activity of at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system;
d) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to said amino acid sequence of (b), wherein said amino acid sequence has a biological activity of at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system; and
e) a nucleic acid sequence that is fully complementary to the nucleic acid sequence of (a), (b), (c), or (d).
3 Assignments
0 Petitions
Accused Products
Abstract
The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
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Citations
55 Claims
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1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
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a) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, and biologically active fragments thereof;
b) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ BD NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ BD NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, and biologically active fragments thereof;
c) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to at least 500 consecutive amino acids of said amino acid sequence of (a), wherein said amino acid sequence has a biological activity of at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system;
d) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to said amino acid sequence of (b), wherein said amino acid sequence has a biological activity of at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system; and
e) a nucleic acid sequence that is fully complementary to the nucleic acid sequence of (a), (b), (c), or (d). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A genetically modified microorganism, wherein said microorganism expresses a PKS system comprising at least one biologically active domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system, wherein said at least one domain of said PUFA PKS system is encoded by a nucleic acid sequence selected from the group consisting of:
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a) a nucleic acid sequence encoding at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system from a Thraustochytrid microorganism;
b) a nucleic acid sequence encoding at least one domain of a PUFA PKS system from a microorganism identified by the following method;
(i) selecting a microorganism that produces at least one PUFA; and
,(ii) identifying a microorganism from (i) that has an ability to produce increased PUFAs under dissolved oxygen conditions of less than about 5% of saturation in the fermentation medium, as compared to production of PUFAs by said microorganism under dissolved oxygen conditions of greater than about 5% of saturation in the fermentation medium;
c) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, and biologically active fragments thereof;
d) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, and biologically active fragments thereof;
e) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to at least 500 consecutive amino acids of an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, and SEQ ID NO;
6;
wherein said amino acid sequence has a biological activity of at least one domain of a PUFA PKS system; and
,f) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, and SEQ ID NO;
32;
wherein said amino acid sequence has a biological activity of at least one domain of a PUFA PKS system;
wherein said microorganism is genetically modified to affect the activity of said PKS system.
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10-44. -44. (canceled)
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45. A genetically modified plant, wherein said plant has been genetically modified to recombinantly express a PKS system comprising at least one biologically active domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system, wherein said domain is encoded by a nucleic acid sequence selected from the group consisting of:
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a) a nucleic acid sequence encoding at least one domain of a polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) system from a Thraustochytrid microorganism;
b) a nucleic acid sequence encoding at least one domain of a PUFA PKS system from a microorganism identified by the following method;
(i) selecting a microorganism that produces at least one PUFA; and
,(ii) identifying a microorganism from (i) that has an ability to produce increased PUFAs under dissolved oxygen conditions of less than about 5% of saturation in the fermentation medium, as compared to production of PUFAs by said microorganism under dissolved oxygen conditions of greater than about 5% of saturation in the fermentation medium;
c) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, and biologically active fragments thereof;
d) a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ BD NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, and biologically active fragments thereof;
e) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to at least 500 consecutive amino acids of an amino acid sequence selected from the group consisting of;
SEQ ID NO;
2, SEQ ID NO;
4, and SEQ ID NO;
6;
wherein said amino acid sequence has a biological activity of at least one domain of a PUFA PKS system; and
,f) a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to an amino acid sequence selected from the group consisting of;
SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
13, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, and SEQ ID NO;
32;
wherein said amino acid sequence has a biological activity of at least one domain of a PUFA PKS system. - View Dependent Claims (46, 47, 48, 49, 50, 51, 52, 53, 54)
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55-135. -135. (canceled)
Specification