Solid medium and method for DNA storage

• US 20050282180A1
• Filed: 12/07/2004
• Published: 12/22/2005
• Est. Priority Date: 10/05/1988
• Status: Abandoned Application

• Current Assignee: Whatman, Inc. (GE Healthcare Technologies, Inc.)
• Original Assignee: Whatman, Inc. (GE Healthcare Technologies, Inc.)
• Inventors: Burgoyne, Leigh A.
• Application Number:
  US11/007,382
• Publication Number:
  US 20050282180A1
• Time in Patent Office: Days
• US Class Current: 435/6.16
• CPC Class Codes: C12M 1/34 Measuring or testing with condition measuring or sensing means, e.g. colony counters
• CPC Class Codes: C12N 15/1003 Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.

Claims

1. A dry solid medium for preserving genetic material from a sample comprising genetic material, wherein the dry solid medium comprises:

a. a fiber matrix; and

b. a surfactant,

i. wherein the surfactant is sorbed on or incorporated into the fiber matrix; and

ii. wherein the surfactant protects genetic material, when a sample comprising genetic material is applied to the dry solid medium, from degradation.

6. The dry solid medium of claim 1, wherein the surfactant comprises an anionic detergent.

7. The dry solid medium of claim 6, wherein the anionic detergent comprises sodium dodecyl sulfate.

8. The dry solid medium of claim 1, wherein the fiber matrix comprises synthetic glass fibers and filaments.

9. The dry solid medium of claim 1, further comprising releasing means for releasing the genetic material from said fiber matrix.

10. The dry solid medium of claim 1, wherein the genetic material comprises DNA.

11. The dry solid medium of claim 1, wherein the genetic material comprises RNA.

2-4. -4. (canceled)

5. A method of preserving genetic material from a sample comprising genetic material, wherein the method comprises:

a. providing a dry solid medium, wherein the dry solid medium comprises;

i. a fiber matrix; and

ii. a surfactant wherein the surfactant is sorbed on or incorporated into the fiber matrix;

b. providing a sample comprising genetic material;

c. contacting the dry solid medium with the sample comprising genetic material; and

d. sorbing the genetic material to the dry solid medium, wherein the genetic material sorbed to the dry solid medium is preserved and protected by the surfactant.

12. The method of claim 5, wherein the surfactant comprises an anionic detergent.

13. The method of claim 12, wherein the anionic detergent comprises sodium dodecyl sulfate.

14. The method of claim 5, wherein the fiber matrix comprises synthetic glass fibers and filaments.

15. The method of claim 5, wherein the genetic material comprises DNA.

16. The method of claim 5, wherein the genetic material comprises RNA.

17. A dry solid medium for preserving DNA from a sample comprising DNA, wherein the dry solid medium comprises:

a. a fiber matrix, wherein the fiber matrix comprises synthetic glass fibers and filaments; and

b. a surfactant,

i. wherein the surfactant is sorbed on or incorporated into the fiber matrix; and

ii. wherein the surfactant protects DNA, when a sample comprising DNA is applied to the dry solid medium, from degradation.

18. The dry solid medium of claim 17, wherein the anionic detergent comprises sodium dodecyl sulfate.

19. A method for preserving RNA from a sample comprising RNA, wherein the method comprises:

a. providing a dry solid medium comprising;

i. a fiber matrix, wherein the fiber matrix comprises synthetic glass fibers and filaments; and

ii. a surfactant,

wherein the surfactant is sorbed on or incorporated into the fiber matrix; and

wherein the surfactant comprises an anionic detergent;

b. providing a sample comprising RNA;

c. contacting the dry solid medium with the sample comprising RNA; and

d. sorbing the RNA to the dry solid medium, wherein the RNA sorbed to the dry solid medium is preserved and protected by the surfactant.

20. The method of claim 19, wherein the anionic detergent comprises sodium dodecyl sulfate.

21. The method of claim 19, wherein the dry solid medium further comprises:

iii. a weak base; and

iv. a chelating agent.

22. The method of claim 21, wherein the weak base comprises Tris and the chelating agent comprises ethylenediamine tetra-acetic acid (EDTA).

23. The method of claim 21, wherein the dry solid medium further comprises:

v. uric acid or a urate salt.