Microarray substrate having patterned thin layer and microarray comprising the same, and methods of producing the microarray substrate and the microarray

• US 20050282204A1
• Filed: 05/31/2005
• Published: 12/22/2005
• Est. Priority Date: 06/16/2004
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Hwang, Kyu-youn, Shim, Jeo-young, Namgoong, Ji-na
• Application Number:
  US11/141,437
• Publication Number:
  US 20050282204A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00731 Saccharides
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 21/45 : using interferometric methods; using Schlieren methods
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

A microarray substrate having a patterned thin layer on a substrate is provided. In the microarray substrate, the substrate has a reflectance different from that of a material of the thin layer, and the patterned thin layer includes a spot region having a thickness at which constructive interference occurs between a first reflected light of irradiated excitation light reflected from the substrate and a second reflected light of irradiated excitation light reflected from the thin layer and a background region having a thickness at which destructive interference occurs between the first reflected light of irradiated excitation light reflected from the substrate and the second reflected light of irradiated excitation light reflected from the thin layer.

Claims

1. A microarray substrate having a patterned thin layer on a substrate, wherein the substrate has a reflectance different from that of a material of the thin layer, and the patterned thin layer includes a spot region having a thickness at which constructive interference occurs between a first reflected light of irradiated excitation light reflected from the substrate and a second reflected light of irradiated excitation light reflected from the thin layer and a background region having a thickness at which destructive interference occurs between the first reflected light of irradiated excitation light reflected from the substrate and the second reflected light of irradiated excitation light reflected from the thin layer.

2. The microarray substrate of claim 1, wherein the substrate is selected from the group consisting of glass, silicon wafer, fused silica, gold, silver, copper, platinum, polystyrene, poly(methylacrylate), and polycarbonate.

3. The microarray substrate of claim 1, wherein the thin layer is selected from the group consisting of silicon dioxide (SiO₂), glass, lithium fluoride (LiF), silicon wafer, fused silica, ceramic, mica, Al₂O₃, TiO₂, ITO, polystyrene, poly(methylacrylate), and polycarbonate.

4. The microarray substrate of claim 1, wherein the reflectance of the substrate is greater than that of the material of the thin layer, the thickness of the spot region satisfies an equation of d=(2m+1)λ

/4n sin θ and

the thickness of the background region satisfies an equation of d=2mλ

/4n sin θ

, in which m is 0 or a positive integer, d is a thickness, n is a reflectance, θ

is an incidence angle of an incident light, and λ

is a wavelength.

5. The microarray substrate of claim 1, wherein the substrate is silicon or glass and the patterned thin layer is SiO₂.

6. The microarray substrate of claim 5, wherein the thickness of the spot region is in a range of 500-1,500 Å

.

7. The microarray substrate of claim 5, wherein the thickness of the background region is in a range of 0-10 Å

or 1,900-2,000 Å

.

8. A microarray comprising the microarray substrate of claim 1.

9. The microarray of claim 8, wherein a biomolecule selected from the group consisting of proteins, nucleic acids, and polysaccharides is immobilized on the spot region.

10. A method of producing the microarray substrate of claim 1, the method comprising:

coating on a substrate a thin layer material having a reflectance different from that of the substrate to form a thin layer; and

patterning the thin layer to form a spot region having a thickness at which constructive interference occurs between a first reflected light of irradiated excitation light reflected from the substrate and a second reflected light of irradiated excitation light reflected from the thin layer and a background region having a thickness at which destructive interference occurs between the first reflected light of irradiated excitation light reflected from the substrate and the second reflected light of irradiated excitation light reflected from the thin layer.

11. A method of producing the microarray of claim 8, the method comprising:

coating on a substrate a thin layer material having a reflectance different from that of the substrate to form a thin layer;

patterning the thin layer to form a spot region having a thickness at which constructive interference occurs between a first reflected light of irradiated excitation light reflected from the substrate and a second reflected light of irradiated excitation light reflected from the thin layer and a background region having a thickness at which destructive interference occurs between the first reflected light of irradiated excitation light reflected from the substrate and the second reflected light of irradiated excitation light reflected from the thin layer; and

immobilizing biomolecules on the spot region.

12. The method of claim 11, wherein the biomolecule is selected from the group consisting of proteins, nucleic acids, and polysaccharides.