Methods and device for analyte characterization
First Claim
1. A method comprising:
- a) obtaining one or more labeled proteins, polypeptides or peptides;
b) passing the labeled proteins, polypeptides or peptides through one or more nanopores;
c) detecting labeled amino acid residues in the labeled proteins, polypeptides or peptides;
d) compiling an amino acid distance map for each type of labeled amino acid; and
e) identifying the protein based on the distance maps.
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Abstract
The methods and apparatus, disclosed herein are of use for sequencing and/or identifying proteins, polypeptides and/or peptides. Proteins containing labeled amino acid residues may be synthesized and passed through nanopores. A detector operably coupled to a nanopore may detect labeled amino acid residues as they pass through the nanopore. Distance maps for each type of labeled amino acid residue may be compiled. The distance maps may be used to sequence and/or identify the protein. Apparatus of use for protein sequencing and/or identification is also disclosed herein. In alternative methods, other types of analytes may be analyzed by the same techniques.
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Citations
30 Claims
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1. A method comprising:
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a) obtaining one or more labeled proteins, polypeptides or peptides;
b) passing the labeled proteins, polypeptides or peptides through one or more nanopores;
c) detecting labeled amino acid residues in the labeled proteins, polypeptides or peptides;
d) compiling an amino acid distance map for each type of labeled amino acid; and
e) identifying the protein based on the distance maps. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An apparatus comprising:
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a) at least one sub-device, each sub-device comprising an first chamber and a second chamber, said first and second chambers separated by sensor layers, the first and second chambers of each sub-device in fluid communication through one or more nanopores; and
b) one or more detectors operably coupled to the nanopores. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method comprising:
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a) contacting one or more cells with a labeled subunit;
b) obtaining one or more copies of a molecule comprising labeled subunits from the cells;
c) passing the labeled molecule through one or more nanopores;
d) detecting labeled subunits on the labeled molecule;
e) compiling a subunit distance map; and
f) identifying the molecule from the distance map. - View Dependent Claims (28, 29, 30)
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Specification