Thin films for controlled protein interaction

• US 20050282925A1
• Filed: 02/23/2005
• Published: 12/22/2005
• Est. Priority Date: 02/23/2004
• Status: Active Grant

• Current Assignee: Florida State University Research Foundation, Inc. (State University System of Florida)
• Original Assignee: Florida State University Research Foundation, Inc. (State University System of Florida)
• Inventors: Salloum, David S., Schlenoff, Joseph B., Sui, Zhijie, Rmaile, Hassan
• Granted Patent: US 8,481,017 B2
• Time in Patent Office: Days
• US Class Current: 523/106
• CPC Class Codes: C02F 1/4693 electrodialysis
• CPC Class Codes: C08L 39/00 Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen; Compositions of derivatives of such polymers
• CPC Class Codes: G02B 1/04 made of organic materials, e.g. plastics G02B1/08 takes precedence

Abstract

A medium for isolating or releasing an electrostatically charged component from or into an aqueous composition. The medium has a polyelectrolyte film on at least one surface of an article wherein the polyelectrolyte film is characterized by an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer. The predominantly positively charged polymer, the predominantly negatively charged polymer or both contain (i) a pH sensitive imidazole repeat unit having a pKa between 3 and 9, or (ii) a redox sensitive repeat unit selected from the group consisting of quaternized bipyridine repeat units, coordinated metal repeat units, pyrrole repeat units, aniline repeat units, thiophene repeat units and combinations thereof having a redox potential between +1.2 volts and −1.2 volts versus a standard hydrogen electrode.

Claims

1. A medium for isolating or releasing an electrostatically charged component from or into an aqueous composition, the medium comprising a polyelectrolyte film on at least one surface of an article other than a capillary tube wherein

the polyelectrolyte film comprises an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer,

the predominantly positively charged polymer, the predominantly negatively charged polymer or both contain (i) a pH sensitive imidazole repeat unit having a pKa between 3 and 9 , or (ii) a redox sensitive repeat unit selected from the group consisting of quaternized bipyridine repeat units, coordinated metal repeat units, pyrrole repeat units, aniline repeat units, thiophene repeat units and combinations thereof having a redox potential between +1.2 volts and −

1.2 volts versus a standard hydrogen electrode.

2. The medium of claim 1 wherein

the imidazole repeat unit has the structure:

wherein each R₁ is independently optionally substituted alkyl, aryl, alkaryl, or heterocyclo, and n is 0 to 2,

the quaternized bipyridine repeat unit has the structure;

wherein R₃ and R₄ are optionally substituted alkyl, aryl, alkaryl, or heterocyclo,

the coordinated metal repeat unit has the structure;

wherein M is osmium, ruthenium, cobalt or iron, and X₁ to X₆ are independently pyridine, bipyridine, terpyridine, nitrile, aceyl acetonate, trisbipyridyl, pyridine carboxylate, pyrazine carboxylate or imidazole,

the pyrrole repeat unit has the structure;

wherein X₇ and X₈ are independently hydrogen, or optionally substituted alkyl or alkoxy,

the aniline repeat unit has the structure;

wherein X₉ is hydrogen, SO₃⁻

or optionally substituted alkyl or alkoxy, and

the thiophene repeat unit has the structure;

wherein X₁₀ and X₁₁ are independently hydrogen, or optionally substituted alkyl or alkoxy.

3. The medium of claim 1 wherein the polyelectrolyte multilayer comprises an imidazole repeat unit having the structure:

wherein each R₁ is independently optionally substituted alkyl, aryl, alkaryl, or heterocyclo, and n is 0 to 2,

4. The medium of claim 1 wherein imidazole ring is bonded to the polyvinyl backbone by a ring carbon atom and each R₁ is hydrogen.

5. The medium of claim 2 or 3 wherein the predominantly positively charged polymer comprises the imidazole repeat unit and the predominantly positively charged polymer also comprises repeat units comprising quaternary ring nitrogen atoms.

6. The medium of claim 5 wherein the ratio of repeat units having quaternized ring nitrogens to the imidazole repeat units in the predominantly positively charged polymer is between 1:

9 and 9;

1, respectively.

7. The medium of claim 1 or 2 wherein article is a membrane, particulate material, or a container having a bottom and a sidewall formation, or a region in a two-dimensional array formed on a substrate.

8. The medium of claim 1 or 2 wherein the predominantly positively charged polymer, the predominantly negatively charged polymer or both contain a redox sensitive repeat unit and the article is selected from the group consisting of beads, fibers and nanotubes.

9. The medium of claim 1 or 2 wherein the polyelectrolyte film comprises carbon fibers or carbon nanotubes.

10. A process for isolating an electrostatically charged component from a first aqueous composition, the process comprising contacting the first aqueous composition with a polyelectrolyte film to sorb the ionized component onto or into the polyelectrolyte film, wherein

the polyelectrolyte film comprises an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer, and

the predominantly positively charged polymer, the predominantly negatively charged polymer or both contain (i) a pH sensitive imidazole repeat unit having a pKa between 3 and 9, or (ii) a redox sensitive repeat unit selected from the group consisting of quaternized bipyridine repeat units, coordinated metal repeat units, pyrrole repeat units, aniline repeat units, thiophene repeat units and combinations thereof having a redox potential between +1.2 volts and −

1.2 volts versus a standard hydrogen electrode.

11. The process of claim 10 wherein the polyelectrolyte film comprises said pH sensitive repeat unit, the ionized component is sorbed from the first aqueous composition onto or into the polyelectrolyte film at a first pH, and the sorbed ionic composition is desorbed from the polyelectrolyte film into a second aqueous composition at a second pH, wherein the composition of the second aqueous composition differs from the composition of the first aqueous material, and the difference between the first and second pH'"'"'s is at least 0.5 pH units.

12. The process of claim 10 wherein the polyelectrolyte film comprises said redox sensitive repeat unit, the ionized component is sorbed from the first aqueous composition onto or into the polyelectrolyte film at a first applied potential, and the sorbed ionic composition is desorbed from the polyelectrolyte film into a second aqueous material at a second applied potential, wherein the composition of the second aqueous composition differs from the composition of the first aqueous composition and the difference between the first and second applied potentials is at least 0.1 volts.

13. The process of claim 11 or 12 wherein the first aqueous composition is an aqueous solution and the ionic composition is dissolved therein.

14. The process of claim 13 wherein the dissolved ionic composition is a dissolved ionic organic composition.

15. The process of claim 14 wherein the dissolved ionic organic composition is selected from the group consisting of polypeptides, nucleic acids, polysaccharides, small organic molecules, and coordination complexes.

16. The process of claim 11 or 12 wherein the ionic composition is an ionized particle selected from the group consisting of viruses, viral fragments, cells, cell fragments, and peptide conjugates.