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Nucleic acid amplification and detection method

  • US 20050287591A1
  • Filed: 08/24/2005
  • Published: 12/29/2005
  • Est. Priority Date: 07/31/2002
  • Status: Abandoned Application
First Claim
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1. A method of determining whether a test sample contains a target nucleic acid comprising a target sequence, said method comprising the steps of:

  • providing a device for amplifying nucleic acids and detecting amplification products, said device comprising, a solid support having a surface, a plurality of species of amplification primer immobilized to said surface, said plurality of species of amplification primer comprising a first amplification primer that comprises a sequence complementary to a first strand of said target nucleic acid, and a plurality of species of detectably labeled hybridization probe immobilized to said surface, said plurality of species of detectably labeled hybridization probe comprising a first hybridization probe that comprises a detectable label and a sequence of bases complementary to said target sequence;

    contacting said plurality of species of amplification primer and said plurality of species of detectably labeled hybridization probe with said test sample;

    synthesizing in an in vitro amplification reaction multiple copies of said target sequence and the complement thereof by a process comprising enzymatic extension of said first amplification primer immobilized to said surface, whereby there are produced soluble amplification products if said test sample contained said target nucleic acid; and

    detecting hybridization of said soluble amplification products to said first hybridization probe immobilized to said surface, thereby determining that said test sample contained said target nucleic acid, wherein each of said plurality of species of detectably labeled hybridization probe and said plurality of species of amplification primer is in fluid communication with the others during the synthesizing and detecting steps.

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