RNA interference compositions and screening methods for the identification of novel genes and biological pathways
First Claim
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1. A method of reducing nonspecific suppression of gene expression in response to an introduced double-stranded polynucleotide, comprising:
- (a) introducing an agent that attenuates a pathway of nonspecific suppression into a cell; and
(b) introducing an RNAi molecule that induces nonspecific suppression of gene expression into the cell, wherein said agent reduces nonspecific suppression of gene expression induced by said double-stranded polynucleotide.
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Abstract
The present invention provides compositions and methods for enhancing RNA interference and facilitating the use of long RNA interference molecules. Accordingly, the invention includes a variety of novel applications of RNA interference, including methods related to screening RNA interference molecules using reporter genes to identify biological pathways, genes, therapeutic compounds and biomarkers.
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Citations
20 Claims
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1. A method of reducing nonspecific suppression of gene expression in response to an introduced double-stranded polynucleotide, comprising:
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(a) introducing an agent that attenuates a pathway of nonspecific suppression into a cell; and
(b) introducing an RNAi molecule that induces nonspecific suppression of gene expression into the cell, wherein said agent reduces nonspecific suppression of gene expression induced by said double-stranded polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification