Separation apparatus and separation method

• US 20060000772A1
• Filed: 11/28/2003
• Published: 01/05/2006
• Est. Priority Date: 11/29/2002
• Status: Abandoned Application

• Current Assignee: NEC Corporation
• Original Assignee: NEC Corporation
• Inventors: Sano, Toru, Asogawa, Minoru, Iguchi, Noriyuki, Iida, Kazuhiro, Someya, Hiroko, Baba, Masakazu, Kawaura, Hisa
• Application Number:
  US10/536,798
• Publication Number:
  US 20060000772A1
• Time in Patent Office: Days
• US Class Current: 210/635
• CPC Class Codes: B01D 67/0062 by micromachining techniques, e.g. using masking and etching steps, photolithography
• CPC Class Codes: B01D 69/00 Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
• CPC Class Codes: B01D 69/141 Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
• CPC Class Codes: B01D 71/027 Silicium oxide
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 1/405 by adsorption or absorption

Abstract

A channel (103) is formed in a substrate (101), and a portion of the channel (103) is provided with a separating portion (107). A number of pillars are formed in the separating portion (107), and an adsorptive substance layer having an adsorptive substance, which exhibits a specific interaction for a specific substance, immobilized on the surface thereof, is formed. Once a sample is introduced into the channel (103), the specific substance is adsorbed on the adsorptive substance layer to be separated from other components. After washing the inside of the channel (103) with a buffer solution, the specific substance is desorbed from the adsorptive substance layer by flowing a eluting solution through the channel (103) and the specific substance is recovered.

Claims

1. A separation apparatus, including:

a substrate;

a channel provided in said substrate and for flowing sample in said channel;

a separating portion provided in said channel and for separating a specific substance in said sample; and

a fine channel provided in said separating portion and having a width that is smaller than that of said channel,

wherein a layer of an adsorptive substance is formed in said separating portion, said adsorptive substance selectively adsorbing or binding to said specific substance.

3. The separation apparatus according to claim 1 or 2, wherein electrodes are provided in said separating portion and said channel, and said separation apparatus further comprises an electrical voltage applying unit that provides electrical voltage between said electrodes.

4. The separation apparatus according to any one of claims 1 or 2, wherein a protruding portion is provided in said separating portion, and an electrode is formed in said protruding portion.

5. The separation apparatus according to any one of claims 1 or 2, wherein a combination of said specific substance and said adsorptive substance is any one of:

an antigen and an antibody;

an enzyme and a substrate;

an enzyme and a substrate derivative;

an enzyme and an inhibitor;

a sugar and a lectin;

a DNA and a DNA;

a DNA and an RNA;

a protein and a nucleic acid;

a metal and a protein and a ligand and a receptor.

6. The separation apparatus according to any one of claims 1 or 2, wherein said adsorptive substance is provided through a spacer on the surface of said substrate.

9. A mass spectrometry system, comprising:

a separating unit that separates a biological sample according to molecular size or a property of the biological sample;

a pretreatment unit that conducts a pretreatment including an enzymatic digestion treatment of the sample separated by said separating unit;

a drying unit that dries the pretreated sample; and

a mass spectrometry unit that achieves a mass spectrometry of the sample after drying,

wherein said separating unit contains the separation apparatus according to any one of claim 1 or 2.

2. A separation apparatus, including:

a substrate;

a channel provided in said substrate and for flowing sample in said channel;

a separating portion provided in said channel and for separating a specific substance in said sample; and

a protruding portion provided in said separating portion,

wherein a layer of an adsorptive substance is formed in said separating portion, said adsorptive substance selectively adsorbing or binding to said specific substance.

7. A separation method, comprising:

in a separating portion of a separation apparatus including a channel provided in a substrate, a separating portion provided in said channel and a fine channel provided in said separating portion and having a width that is smaller than that of said channel,

introducing a liquid containing an adsorptive substance into said channel while applying an electrical voltage of different polarity than that of said adsorptive substance to said separating portion to cause an adsorption onto said separating portion, said adsorptive substance being capable of selectively adsorbing or binding to a separating target substance;

introducing a sample containing said separating target substance into said channel to cause a selective adsorption or binding to said adsorptive substance; and

introducing an eluting solution into said channel to elute and recover said separating target substance, said eluting solution being capable of causing an elution of said separating target substance from said adsorptive substance.

8. A separation method, comprising:

in a separating portion of a separation apparatus including a channel provided in a substrate, a separating portion provided in said channel and a protruding portion provided in said separating portion, introducing a liquid containing an adsorptive substance into said channel while applying an electrical voltage of different polarity than that of said adsorptive substance to said separating portion of said separating apparatus to cause an adsorption onto said separating portion, said adsorptive substance being capable of selectively adsorbing or binding to a separating target substance;

introducing a sample containing said separating target substance into said channel to cause a selective adsorption or binding to said adsorptive substance; and

introducing an eluting solution into said channel to elute and recover said separating target substance, said eluting solution being capable of causing an elution of said separating target substance from said adsorptive substance.