Dispensing systems, software, and related methods

• US 20060002824A1
• Filed: 06/06/2005
• Published: 01/05/2006
• Est. Priority Date: 06/07/2004
• Status: Abandoned Application

• Current Assignee: IRM LLC
• Original Assignee: IRM LLC
• Inventors: Shaw, Christopher M., Downs, Robert Charles, Chang, Jim Yuchen, Mainquist, James Kevin, Micklash, Kenneth J. II
• Application Number:
  US11/146,916
• Publication Number:
  US 20060002824A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00355 peristaltic
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00364 Pipettes
• CPC Class Codes: B01J 2219/00389 Feeding through valves
• CPC Class Codes: B01J 2219/004 Pinch valves
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00695 Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0829 : Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/0481 : squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 : fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 : pinch valves
• CPC Class Codes: B01L 3/021 : Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0241 : Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0293 : for liquids
• CPC Class Codes: B01L 9/52 : Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: B01L 9/523 : for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: G01N 35/08 : using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1002 : Reagent dispensers
• CPC Class Codes: G01N 35/1016 : Control of the volume dispensed or introduced
• CPC Class Codes: G01N 35/1065 : Multiple transfer devices

Abstract

The present invention provides dispensing systems that include peristaltic pumps and other pressure sources for the efficient delivery of accurate volumes of fluidic materials into the wells of multi-well containers or onto substrate surfaces. These systems are typically configured to dispense volumes of fluid having substantially uniform densities. Related computer program products and methods of dispensing fluidic materials are also provided.

Claims

1. A dispensing system, comprising:

at least one peristaltic pump configured to convey at least a first fluidic material into or through at least a portion of at least one conduit when the conduit is operably connected to the peristaltic pump and is in fluid communication with at least a first fluidic material source;

at least one pressure source other than the peristaltic pump, which pressure source is configured to apply pressure in the conduit when the pressure source is operably connected to the conduit such that selected aliquots of the first fluidic material are dispensed from at least one opening in the conduit when the first fluidic material is present in the conduit; and

,

at least one controller operably connected to the pressure source, which controller is configured to control operation of the pressure source to effect dispensing of the first fluidic material from the opening in the conduit when the conduit is in fluid communication with the first fluidic material source.

2. The dispensing system of claim 1, wherein the peristaltic pump comprises a multi-channel peristaltic pump.

3. The dispensing system of claim 1, wherein the controller is operably connected to the peristaltic pump and configured to effect rotation of a roller support of the peristaltic pump in at least one rotational increment that substantially corresponds to an integral multiple of an angular distance disposed between adjacent rollers supported by the roller support such that quantities of the first fluidic material that correspond to the rotational increment are conveyed into or through the conduit when the conduit is operably connected to the peristaltic pump and is in fluid communication with the first fluidic material source.

4. The dispensing system of claim 1, comprising a mounting component to which the peristaltic pump, the pressure source, the controller, and/or another system component is attached.

5. The dispensing system of claim 1, wherein the dispensing system comprises the conduit.

6. The dispensing system of claim 5, comprising at least one waste collection component configured to selectively communicate with the opening in the conduit.

7. The dispensing system of claim 5, comprising a fluid reservoir in fluid communication with the conduit.

8. The dispensing system of claim 5, wherein a substantial portion of the conduit is disposed other than parallel to a Z-axis of the dispensing system.

9. The dispensing system of claim 5, wherein at least a segment of the conduit that comprises the opening is disposed at an angle of between about 0°

and about 90°

relative to a Z-axis of the dispensing system.

10. The dispensing system of claim 5, wherein the opening in the conduit comprises at least one dispensing tip.

11. The dispensing system of claim 5, wherein the opening in the conduit comprises at least one manifold that is configured to fluidly communicate with multiple fluidic material sites.

12. The dispensing system of claim 5, wherein the pressure source comprises one or more pumps.

13. The dispensing system of claim 5, wherein the dispensing system comprises multiple conduits.

14. The dispensing system of claim 13, wherein openings in at least two of the conduits are spaced at a distance from one another to simultaneously fluidly communicate with different wells disposed in at least one multi-well container.

15. The dispensing system of claim 5, wherein at least a segment of the conduit disposed between the opening and the peristaltic pump comprises a conduit coil.

16. The dispensing system of claim 15, wherein at least one coil in the conduit coil is disposed other than parallel to a Z-axis of the dispensing system.

17. The dispensing system of claim 5, wherein the peristaltic pump is operably connected to at least a first conduit and the pressure source is operably connected to at least a second conduit, which first and second conduits fluidly communicate with one another.

18. The dispensing system of claim 17, wherein at least one three-way valve is operably connected to the first conduit, which three-way valve is structured to selectively vent the first conduit.

19. The dispensing system of claim 5, wherein the pressure source is in fluid communication with the conduit.

20. The dispensing system of claim 19, comprising at least one filter operably connected to the conduit.

21. The dispensing system of claim 19, wherein the pressure source comprises a pressurized gas source and/or a pressurized second fluidic material source.

22. The dispensing system of claim 21, wherein the second fluidic material source comprises at least one buffer.

23. The dispensing system of claim 5, wherein the pressure source is operably connected to the conduit via at least one solenoid valve that regulates pressure applied by the pressure source.

24. The dispensing system of claim 23, wherein the controller is operably connected to the solenoid valve, which controller is configured to control operation of the solenoid valve to effect regulation of the applied pressure.

25. The dispensing system of claim 5, wherein at least one port is disposed through at least one wall of the conduit, which port communicates with at least one cavity disposed through the conduit.

26. The dispensing system of claim 25, wherein the port comprises a length of about 5 mm or less.

27. The dispensing system of claim 25, wherein a region of the conduit that comprises the port comprises a fluid junction block.

28. The dispensing system of claim 25, wherein the port is disposed between the peristaltic pump and the pressure source in the conduit.

29. The dispensing system of claim 25, wherein at least one gas valve is operably connected to the port, which gas valve regulates gas flow into the conduit through the port when the gas valve is operably connected to at least one pressurized gas source.

30. The dispensing system of claim 29, wherein the gas valve comprises a plunger comprising a compliant seal material that forms a face seal with the port when the plunger pushes the compliant seal material into contact with the port.

31. The dispensing system of claim 29, wherein the gas valve is operably connected to the pressurized gas source, which pressurized gas source flows gas to the gas valve at a pressure of between about zero pounds per square inch and about 10 pounds per square inch.

32. The dispensing system of claim 31, wherein the gas comprises air, nitrogen, helium, or argon.

33. The dispensing system of claim 29, wherein at least one air table is operably connected to the gas valve, which air table is configured to effect operation of the gas valve.

34. The dispensing system of claim 33, wherein the controller is operably connected to the air table, which controller is configured to control operation of the air table to effect regulation of gas flow into the conduit through the port when the gas valve is operably connected to the pressurized gas source.

35. The dispensing system of claim 1, comprising at least one pinch valve configured to regulate conveyance of fluidic materials through the conduit when the conduit is operably connected to the pinch valve.

36. The dispensing system of claim 35, wherein at least one air table is operably connected to the pinch valve, which air table is configured to effect operation of the pinch valve.

37. The dispensing system of claim 36, wherein the controller is operably connected to the air table, which controller is configured to control operation of the air table to effect regulation of fluidic material conveyance through the conduit when the conduit is operably connected to the pinch valve.

38. The dispensing system of claim 1, wherein the dispensing system comprises the first fluidic material source.

39. The dispensing system of claim 38, wherein the first fluidic source comprises one or more of:

beads, cells, enzymes, or reagents.

40. The dispensing system of claim 38, wherein at least one fluid agitation mechanism is operably connected to the first fluidic material source.

41. The dispensing system of claim 1, comprising at least one positioning component operably connected to the controller, which positioning component is configured to moveably position one or more conduits and/or one or more fluidic material sites relative to one another.

42. The dispensing system of claim 41, wherein the positioning component comprises at least one X/Y-axis linear motion component operably connected to at least one control drive that controls movement of the X/Y-axis linear motion component along an X-axis and a Y-axis of the dispensing system.

43. The dispensing system of claim 41, wherein the controller is operably connected to the pressure source and is configured to simultaneously effect application of pressure in the conduits from the pressure source and moveably position the conduits and/or the fluidic material sites relative to one another such that volumes of fluid are conveyed from the conduits synchronous with the relative movement of the conduits and/or the fluidic material sites.

44. The dispensing system of claim 41, wherein the positioning component comprises at least one Z-axis linear motion component comprising at least one conduit support head that is configured to support at least segments of the conduits and that moves along a Z-axis of the dispensing system.

45. The dispensing system of claim 41, wherein the positioning component comprises at least one object holder that is structured to support at least one fluidic material site.

46. The dispensing system of claim 41, comprising at least one cleaning component operably connected to the controller, which cleaning component is configured to clean at least segments of the conduits when the conduits are operably connected to the positioning component and the positioning component moves the conduit segments at least proximal to the cleaning component.

47. The dispensing system of claim 46, wherein the cleaning component comprises at least one vacuum chamber comprising at least one orifice into or proximal to which the positioning component moves the conduit segments such that an applied vacuum removes adherent material from at least external surfaces of the conduit segments.

48. The dispensing system of claim 1, comprising at least one detector configured to detect detectable signals produced in fluidic materials.

49. The dispensing system of claim 48, wherein the controller is operably connected to the detector, which controller is configured to control the detector to effect detection of the detectable signals.

50. A computer program product comprising a computer readable medium having one or more logic instructions for:

operating at least one peristaltic pump to effect conveyance of at least a first fluidic material into at least one conduit through at least a first opening of the conduit; and

,

operating at least one pressure source other than the peristaltic pump to effect application of pressure on the first fluidic material in the conduit such that at least one aliquot of the first fluidic material is dispensed from at least a second opening of the conduit.

51. The computer program product of claim 50, comprising at least one logic instruction for:

receiving one or more input parameters selected from the group consisting of;

(i) a quantity of the first fluidic material to be conveyed to a fluidic material site;

(ii) an initial density of the first fluidic material;

(iii) a quantity of a second fluidic material to be added to the first fluidic material to modify a density of the first fluidic material;

(iv) a quantity of gas to convey into the conduit to separate the first fluidic material from a second fluidic material; and

(v) a fluidic material site format.

52. The computer program product of claim 50, comprising at least one logic instruction for:

operating at least one valve operably connected to the conduit to effect regulation of material conveyance into and/or out of the conduit.

53. The computer program product of claim 50, comprising at least one logic instruction for:

operating at least one X/Y-axis linear motion component and/or at least one Z-axis motion component to effect movement of one or more other components attached to or positioned on the X/Y-axis linear motion component or the Z-axis motion component.

54. A method of dispensing a fluidic material, the method comprising:

(a) conveying at least a first fluidic material into at least one conduit through at least a first opening of the conduit using at least one peristaltic pump; and

,

(b) applying pressure on the first fluidic material in the conduit using at least one pressure source other than the peristaltic pump such that at least one aliquot of the first fluidic material is dispensed from at least a second opening of the conduit.

55. The method of claim 54, comprising dispensing the aliquot of the first fluidic material unto a wall of a container.

56. The method of claim 54, comprising dispensing multiple aliquots of the first fluidic material during (b).

57. The method of claim 54, comprising repeating (a) and (b).

58. The method of claim 54, comprising restricting fluidic material conveyance in the conduit directed towards the peristaltic pump during (b).

59. The method of claim 54, comprising conveying a gas into the conduit to purge fluidic materials from at least one segment of the conduit prior to (a).

60. The method of claim 54, comprising moveably positioning at least one fluidic material site relative to the second opening.

61. The method of claim 54, comprising detecting one or more detectable signals produced in the conduit and/or in the aliquot of the first fluidic material.

62. The method of claim 54, comprising performing at least one synthesis reaction or assay using one or more components in the aliquot of the first fluidic material after (b).

63. The method of claim 54, comprising performing (a) and (b) substantially simultaneously with one another.

64. The method of claim 54, wherein the first fluidic material comprises one or more of:

beads, cells, enzymes, or reagents.

65. The method of claim 54, wherein at least a segment of the conduit comprises a non-vertical flow path to prevent one or more components of the first fluidic material from settling proximal to the second opening.

66. The method of claim 54, comprising conveying at least a second fluidic material through one or more segments of the conduit using the pressure source such that the second fluidic material expels the aliquot of the first fluidic material from the second opening of the conduit during (b).

67. The method of claim 66, wherein the second fluidic material comprises a buffer.

68. The method of claim 66, comprising diluting the first fluidic material with the second fluidic material prior to or substantially simultaneously with (b).

69. The method of claim 66, comprising conveying a gas into the conduit through a port to form a gap between the first and second fluidic materials to prevent the first and second fluidic materials from mixing with one another.

70. A method of dispensing aliquots of fluidic materials having substantially uniform densities, the method comprising conveying selected aliquots of at least one fluidic material from at least one dispensing tip that fluidly communicates with at least one conduit through which the fluidic material is conveyed, which conduit comprises a non-vertical flow path such that components in the fluidic material are prevented from settling proximal to the dispensing tip prior to being dispensed, thereby dispensing the aliquots of fluidic materials having substantially uniform densities.

71. A method of dispensing a fluidic material, the method comprising:

(a) providing a dispensing system having a fluid junction block comprising;

(i) at least a portion of a first conduit that fluidly communicates with a first fluidic material source;

(ii) at least a portion of a second conduit having;

(I) at least first and second openings; and

(II) at least one port disposed through a wall of the second conduit, which port communicates with a cavity disposed through the second conduit,

wherein the first conduit intersects and fluidly communicates with the second conduit between the port and the second opening of the second conduit;

(b) conveying a volume of a second fluidic material through the first opening of the second conduit proximal to the port;

(c) restricting fluidic material conveyance through the first opening of the second conduit and through the first conduit;

(d) conveying at least one gas into the second conduit through the port to purge fluidic materials from the second conduit downstream from the port through the second opening of the second conduit;

(e) restricting fluidic material conveyance through the first opening of the second conduit and gas conveyance through the port;

(f) conveying a volume of a first fluidic material from the first fluidic material source through the first conduit and into the second conduit proximal to and downstream from the intersection of the first and second conduits such that a volume of the gas is disposed between the first and second fluidic materials in the second conduit;

(g) restricting fluidic material conveyance through the first conduit and gas conveyance through the port; and

,

(h) applying pressure to the second fluidic material in the second conduit such that at least one selected aliquot of the first fluidic material is dispensed from the second opening of the second conduit.