Ii-key/antigenic epitope hybrid peptide vaccines

• US 20060002947A1
• Filed: 01/11/2005
• Published: 01/05/2006
• Est. Priority Date: 09/14/1999
• Status: Active Grant

• Current Assignee: Nugenerex Immuno-Oncology Incorporated (Generex Biotechnology Corp.)
• Original Assignee: Antigen Express, Inc. (Generex Biotechnology Corp.)
• Inventors: Xu, Minzhen, Humphreys, Robert
• Granted Patent: US 9,289,487 B2
• Time in Patent Office: Days
• US Class Current: 424/185.100
• CPC Class Codes: A61K 2039/57 characterised by the type of response, e.g. Th1, Th2
• CPC Class Codes: A61K 2039/64 characterised by the architecture of the carrier-antigen complex, e.g. repetition of carrier-antigen units
• CPC Class Codes: A61K 39/385 Haptens or antigens, bound to carriers
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/04 Immunostimulants

Abstract

Disclosed is an antigen presentation enhancing hybrid polypeptide which includes three elements. The first element is an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO: 1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity. The second element is a chemical structure covalently linking the N-terminal element described above to the MHC Class II-presented epitope described below. The chemical structure is a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the chemical structure being selected from the group consisting of: i) immunologically neutral chemical structures, ii) a MHC Class I epitope or a portion thereof, and/or iii) an antibody-recognized determinant or a portion thereof. Finally, the enhancing antigen presentation enhancing hybrid polypeptide includes a C-terminal element comprising an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule.

Claims

1. An antigen presentation enhancing hybrid polypeptide comprising the following elements:

a) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

1 and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

b) a chemical structure covalently linking the N-terminal element of step a) to the MHC Class II-presented epitope of element c), the chemical structure being a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the chemical structure being selected from the group consisting of;

i) immunologically neutral chemical structures;

ii) a MHC Class I epitope or a portion thereof; and

/or

iii) an antibody-recognized determinant or a portion thereof; and

c) a C-terminal element comprising an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule.

2. The antigen presentation enhancing hybrid polypeptide of claim 1 wherein the modifications of element a) are selected from the group consisting of:

a) deletion of amino acids from the C-terminus;

b) protection of the N-terminus;

c) N-terminal extensions; and

d) amino acid substitutions.

3. The antigen presentation enhancing hybrid polypeptide of claim 2 wherein amino acid substitutions include the substitution of a peptidomimetic structure for an amino acid residue.

4. The antigen presentation enhancing hybrid polypeptide of claim 2 wherein the amino acid substitutions to element a) exclude the substitution of amino acid residues aspartate or glutamate for an amino acid found in a wild-type mammalian Ii-Key sequence (SEQ ID NO. 1).

5. The antigen presentation enhancing hybrid polypeptide of claim 2 wherein the amino acid substitutions in element a) include the introduction of a D-isomer amino acid in place of a wild-type amino acid residue.

6. The antigen presentation enhancing hybrid polypeptide of claim 2 wherein the amino acid substitutions in element a) include the introduction of an N-methyl amino acid in place of a wild-type amino acid residue.

7. The antigen presentation enhancing hybrid polypeptide of claim 2 wherein the amino acid substitutions in element a) include the introduction of an L-isomer amino acid or modified L-isomer amino acid.

8. The antigen presentation enhancing hybrid polypeptide of claim 7 wherein the modified L-isomer amino acid in element a) is selected from the group consisting of:

N-methyl leucine;

L-citrulline;

L-homoarginine;

L-ornithine;

L-hydroxyproline;

p-chloro-phenylalanine;

p-fluoro-phenylalanine;

p-nitro-phenylalanine;

α

-amino-4-phenylbutyrate;

β

-thienylalanine;

d-bromo-tyrosine;

di-iodo-tyrosine;

β

-1-napthyl-alanine;

β

-2-napthyl-alanine;

1,2, 3,4-tetrahydroisoquinoline-3-carboxylic acid; and

1,2,3,4-tetrahydroisoquinoline-7-hydroxy-3-carboxylic acid.

9. The antigen presentation enhancing hybrid polypeptide of claim 1 wherein the intervening chemical structure of element b) comprises an MHC Class I-presented epitope, or a portion thereof.

10. The antigen presentation enhancing hybrid polypeptide of claim 1 wherein the C-terminal element further comprises a peptidyl element selected from the group consisting of:

i) an MHC Class I-presented epitope or a portion thereof, and/or

ii) an antibody-recognized determinant or a portion thereof.

11. The antigen presentation enhancing hybrid polypeptide of claim 1 wherein the C-terminal element further comprises an MHC Class I-presented epitope, or a portion thereof, the amino acid residues comprising the MHC Class I-presented epitope or portion thereof being constituent residues of the MHC Class II-presented epitope.

12. The antigen presentation enhancing hybrid polypeptide of claim 1 wherein the C-terminal element further comprises an antibody-recognized determinant, or a portion thereof, the amino acid residues comprising the antibody-recognized determinant or a portion thereof being constituent residues of the MHC Class II-presented epitope.

13. The antigen presentation enhancing hybrid polypeptide of claim 1 which contains one or more MHC Class II-presented epitopes, one or more MHC Class I-presented epitope, and one or more antibody-recognized determinant.

14. An isolated nucleic acid sequence encoding an antigen presentation enhancing hybrid polypeptide comprising the following elements:

a) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

     1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

b) a chemical structure covalently linking the N-terminal element of step a) to the MHC Class II-presented epitope of element c), the chemical structure being a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the chemical structure being selected from the group consisting of;

i) immunologically neutral chemical structures;

ii) a MHC Class I epitope or a portion thereof; and

/or

iii) an antibody-recognized determinant or a portion thereof; and

c) an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule.

15. The antigen presentation enhancing hybrid polypeptide of claim 14 wherein the C-terminal element further comprises a peptidyl element selected from the group consisting of:

i) an MHC Class I-presented epitope or a portion thereof, and/or

ii) an antibody-recognized determinant or a portion thereof.

16. The isolated nucleic acid of claim 14 wherein the modifications of element a) are selected from the group consisting of:

a) deletion of amino acids from the C-terminus;

b) N-terminal extensions; and

c) amino acid substitutions.

17. The isolated nucleic acid of claim 16 wherein the amino acid substitutions to element a) exclude the substitution of amino acid residues aspartate or glutamate for an amino acid found in a wild-type mammalian Ii-Key sequence (SEQ ID NO:

1).

18. The isolated nucleic acid of claim 14 wherein the C-terminal element further comprises an MHC Class I-presented epitope, or a portion thereof, the amino acid residues comprising the MHC Class I-presented epitope or portion thereof being constituent residues of the MHC Class II-presented epitope.

19. The isolated nucleic acid of claim 14 wherein the C-terminal element further comprises an antibody-recognized determinant, or a portion thereof, the amino acid residues comprising the antibody-recognized determinant or a portion thereof being constituent residues of the MHC Class II-presented epitope.

20. A method for enhancing presentation of an MHC Class II-presented antigenic peptide to a T-lymphocyte, the method comprising:

a) providing an antigen presentation enhancing hybrid polypeptide comprising;

i) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

1 and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

ii) a C-terminal element comprising an MHC Class II-presented epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule; and

iii) an intervening chemical structure covalently linking the N-terminal and C-terminal elements of the hybrid, the chemical structure being a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the intervening chemical structure being selected from the group consisting of;

1) immunologically neutral chemical structures;

2) a MHC Class I epitope or a portion thereof; and

/or

3) an antibody-recognized determinant or a portion thereof; and

b) contacting the antigen presentation enhancing hybrid polypeptide of step a), under physiological conditions, with the following components, thereby enhancing presentation of the MHC Class II-presented antigenic peptide to the T-lymphocyte;

i) an antigen-presenting cell expressing MHC Class II molecules which are capable of presenting the step a) ii) antigenic epitope to a T-lymphocyte; and

ii) a T lymphocyte which is responsive to the MHC Class II-presented epitope of element a) ii) when presented by MHC Class II molecules expressed by the antigen presenting cell of step b) i).

21. The method of claim 20 wherein the cells of step b) are provided by a donor individual and, following the manipulation of step b), the cells are reinfused into the donor individual in an ex vivo therapy protocol.

22. The method of claim 20 wherein the C-terminal element further comprises an MHC Class I-presented epitope, or a portion thereof, and the incubation mixture of step b) further comprises a T lymphocyte responsive to the MHC Class I-presented epitope.

23. The method of claim 22 wherein the amino acid residues comprising the MHC Class I-presented epitope, or portion thereof, are constituent residues of the MHC Class II-presented epitope.

24. The method of claim 20 wherein the C-terminal element further comprises an antibody-recognized determinant, or a portion thereof, and the incubation mixture of step b) further comprises a B lymphocyte which expresses immunoglobulin receptors recognizing the antibody-recognized determinant.

25. The method of claim 24 wherein the amino acid residues comprising the antibody-recognized determinant, or a portion thereof, are constituent residues of the MHC Class II-presented epitope.

26. The method of claim 20 wherein the T lymphocyte of step c) has a cytokine release pattern ascribed to a regulatory set of T lymphocytes.

27. The method of claim 26 wherein the regulatory set of T lymphocytes is the TH1 set.

28. The method of claim 26 wherein the regulatory set of T lymphocytes is the TH2 set.

29. A method for immunizing a mammal, thereby increasing immunological sensitivity toward one or more epitopes/determinants which are associated with a pathological condition, the method comprising:

a) providing an antigen presentation enhancing hybrid polypeptide comprising;

i) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

     1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

ii) a C-terminal element comprising an MHC Class II-presented epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule; and

iii) an intervening chemical structure covalently linking the N-terminal and C-terminal elements of the hybrid, the chemical structure being a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the intervening chemical structure being selected from the group consisting of;

1) immunologically neutral chemical structures;

2) a MHC Class I epitope or a portion thereof; and

/or

3) an antibody-recognized determinant or a portion thereof; and

b) administering the antigen presentation enhancing hybrid polypeptide of step a) to a patient in an amount effective to stimulate an immune response.

30. The method of claim 29 wherein the C-terminal element of the antigen presentation enhancing hybrid polypeptide further comprises an MHC Class I-presented epitope, or a portion thereof.

31. The method of claim 30 wherein the amino acid residues comprising the MHC Class I-presented epitope or portion thereof being constituent residues of the MHC Class II-presented epitope.

32. The method of claim 29 wherein the C terminal element of the antigen presentation enhancing hybrid polypeptide further comprises an antibody-recognized determinant, or a portion thereof.

33. The method of claim 32 wherein the amino acid residues comprising the antibody-recognized determinant or a portion thereof being constituent residues of the MHC Class II-presented epitope.

34. A method for immunizing a mammal, thereby increasing immunological sensitivity toward one or more epitopes/determinants which are associated with a pathological condition, the method comprising:

a) providing an expressible nucleic acid sequence encoding an antigen presentation enhancing hybrid polypeptide, the antigen presentation enhancing hybrid polypeptide comprising;

i) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

     1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

ii) a C-terminal element comprising an MHC Class II-presented epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule; and

iii) an intervening peptidyl segment linking the N-terminal and C-terminal elements of the hybrid, the peptidyl segment having a length of up to 20 amino acids, the intervening chemical structure being selected from the group consisting of;

1) immunologically neutral chemical structures;

2) a MHC Class I epitope or a portion thereof; and

/or

3) an antibody-recognized determinant or a portion thereof; and

b) administering the expressible nucleic acid sequence of step a) to a patient in an amount effective to stimulate an immune response.

35. The method of claim 34 wherein the C-terminal element of the antigen presentation enhancing hybrid polypeptide further comprises an MHC Class I-presented epitope, or a portion thereof.

36. The method of claim 35 wherein the amino acid residues comprising the MHC Class I-presented epitope, or portion thereof, are constituent residues of the MHC Class II-presented epitope.

37. The method of claim 34 wherein the C-terminal element of the antigen presentation enhancing hybrid polypeptide further comprises an antibody-recognized determinant, or a portion thereof.

38. The method of claim 37 wherein the amino acid residues comprising the antibody-recognized determinant, or a portion thereof, are constituent residues of the MHC Class II-presented epitope.

39. An antigen presentation enhancing hybrid polypeptide comprising the following elements:

a) an N-terminal element consisting essentially of 4-16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO;

     1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity;

b) a chemical structure covalently linking the N-terminal element of step a) to the MHC Class Ii-presented epitope of element c), the chemical structure being a covalently joined group of atoms which when arranged in a linear fashion forms a flexible chain which extends up to the length of 20 amino acids likewise arranged in a linear fashion, the chemical structure being selected from the group consisting of;

1) immunologically neutral chemical structures;

2) a MHC Class I epitope or a portion thereof; and

/or

3) an antibody-recognized determinant or a portion thereof; and

c) a C-terminal element comprising an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule, the C-terminal element further comprising a peptidyl element selected from the group consisting of;

i) an MHC Class I-presented epitope or a portion thereof, and/or

ii) an antibody-recognized determinant or a portion thereof.