Electrocompetent cells prepackaged for electroporation
First Claim
1. A sterilized electroporation cell cuvette containing frozen electrocompetent cells.
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Abstract
Electrocompetent cells are prepackaged in a sterilized cuvette, and the cell contents frozen, such that electroporation can be performed simply by first thawing the cuvette contents, then electrically connecting the cuvette to a power source suitable for electroporation. The cuvette contains, in addition to the cells, a suspending medium, and optionally also a cryoprotectant for the cells. This invention also resides in a process for preparing electroporation cells for use, or alternatively for shipping or transport, comprising forming a suspension of the cells in a suspending medium preferably comprising a cryoprotectant, placing the cell suspension in a sterilized electroporation cuvette, and quickly freezing the cells while in the cuvette. The cells are then stored, and/or shipped or transported under subzero (Celsius) temperatures for subsequent use.
25 Citations
18 Claims
- 1. A sterilized electroporation cell cuvette containing frozen electrocompetent cells.
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10. A process for preparing electroporation cells for use comprising
(a) forming a suspension of the cells in a suspending medium optionally comprising a cryoprotectant, (b) placing the cell suspension in a sterilized electroporation cuvette, and (c) quickly freezing the cells in the cuvette.
Specification