Exploring fluorophore microenvironments
First Claim
1. A method for detecting a presence of one or more target analytes in particular regions of interest of one or more samples, the method comprising:
- providing one or more samples including a plurality of objects and one or more target analytes, wherein at least some of the target analytes are labeled with a fluorophore and are bound to at least some of the objects in the one or more samples;
illuminating the one or more samples with fluorescence inducing light;
collecting fluorescent light from one or more regions of the one or more samples;
performing at least one anisotropy measurement of the one or more samples to identify one or more regions of interest where one or more target analytes are bound to the objects; and
analyzing the collected fluorescent light from the regions of interest to determine a presence of target analytes that are bound to the objects in the one or more samples.
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Abstract
Methods, apparatus, and system, implementing and using techniques for detecting a presence of one or more target analytes in particular regions of interest of one or more samples. One or more samples including objects and one or more target analytes are provided. Some of the target analytes are labeled with a fluorophore and are bound to some of the objects in the samples. The samples are illuminated with fluorescence inducing light and fluorescent light is collected from one or more regions of the one or more samples. At least one anisotropy measurement of the samples is performed to identify regions of interest where one or more target analytes are bound to the objects. The collected fluorescent light from the regions of interest is analyzed to determine a presence of target analytes that are bound to the objects in the one or more samples.
16 Citations
19 Claims
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1. A method for detecting a presence of one or more target analytes in particular regions of interest of one or more samples, the method comprising:
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providing one or more samples including a plurality of objects and one or more target analytes, wherein at least some of the target analytes are labeled with a fluorophore and are bound to at least some of the objects in the one or more samples;
illuminating the one or more samples with fluorescence inducing light;
collecting fluorescent light from one or more regions of the one or more samples;
performing at least one anisotropy measurement of the one or more samples to identify one or more regions of interest where one or more target analytes are bound to the objects; and
analyzing the collected fluorescent light from the regions of interest to determine a presence of target analytes that are bound to the objects in the one or more samples. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification