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Preparation of defined highly labeled probes

  • US 20060003333A1
  • Filed: 11/19/2003
  • Published: 01/05/2006
  • Est. Priority Date: 11/19/2002
  • Status: Abandoned Application
First Claim
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1. A method for producing a single-stranded unitized nucleic acid probe comprising the acts of:

  • (a) contacting an oligonucleotide primer having a 5′

    recognition end having a length of between about 6 to 50 nucleotides and having a 3′

    priming end having a length of between about 6 to 50 nucleotides with a fixed-size template having a length between 101 and about 10,000 nucleotides under reaction conditions conducive to transcribing a unitized transcript from the fixed-size template; and

    (b) labeling the unitized transcript with at least one detectable molecule, thereby producing a unitized nucleic acid probe.

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